FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151502

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151502
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2985
Total Bases432.6 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC62921.072026800670017No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC47015.745393634840871No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC45015.07537688442211No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC38212.797319932998324No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG31910.68676716917923No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG943.149078726968174No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT612.0435510887772192No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG230.7705192629815745No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT220.7370184254606365No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA110.3685092127303182No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA100.33500837520938026No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA90.3015075376884422No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA80.2680067001675042No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA70.23450586264656617No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT70.23450586264656617No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA60.20100502512562815No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA60.20100502512562815No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA60.20100502512562815No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA60.20100502512562815No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA60.20100502512562815No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT60.20100502512562815No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA60.20100502512562815No Hit
GGCACTAGAATGGACCTCTCCCTCATCACTACATTATTTCAATTAAATAC50.16750418760469013No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA50.16750418760469013No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA50.16750418760469013No Hit
AAATACCGTTAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT50.16750418760469013No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA50.16750418760469013No Hit
AAATACCGTTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAAC50.16750418760469013No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAA50.16750418760469013No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAGTAAGACGTGTAGAACCATGT40.1340033500837521No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT40.1340033500837521No Hit
AAATACCGTTCGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT40.1340033500837521No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGAAAGCCAATGGGAAA40.1340033500837521No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT40.1340033500837521No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATCGCTGCTGGAACC30.10050251256281408No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCCTGCAAATTGCTGCTGGAACC30.10050251256281408No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGATGCTGGAACC30.10050251256281408No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA30.10050251256281408No Hit
GGCACTAGAATGGACCTCTTCGTCATCACTAAATTATTTCAATTAAATAC30.10050251256281408No Hit
GGCACTAGAATGGACCCTTCCTCATCACTACATTATTTCAATTAAATACA30.10050251256281408No Hit
AAATACCGTTAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT30.10050251256281408No Hit
AAATACCGTTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAAC30.10050251256281408No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT30.10050251256281408No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA30.10050251256281408No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA30.10050251256281408No Hit
AATACCGTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT30.10050251256281408No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA30.10050251256281408No Hit
GGCACTAGAATGGACCTCTTTCTCATCACTACATTATTTCAATTAAATAC30.10050251256281408No Hit
AAATACCGTTCAGAGCTGCCACGGCGTCTCCAGACCCAGCTCCGGCCGGG30.10050251256281408No Hit
AAATACCGTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT30.10050251256281408No Hit
AAATACCGTTAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT30.10050251256281408No Hit
GGCACTAGAATGGACCTCCTCCTCATCACTACATTATTTCAATTAAATAC30.10050251256281408No Hit
GGCACTAGAATGGACCTCTTCTCATCACTACATTATTTCAATTAAATACA30.10050251256281408No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)