FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151504

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151504
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2152
Total Bases311.9 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC69932.48141263940521No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC35016.263940520446095No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG22210.315985130111525No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC1868.643122676579926No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC1818.41078066914498No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG863.9962825278810405No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT291.3475836431226766No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA160.7434944237918215No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA140.6505576208178439No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG140.6505576208178439No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA100.4646840148698885No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA90.4182156133828996No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA90.4182156133828996No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA80.37174721189591076No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA80.37174721189591076No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA60.2788104089219331No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA60.2788104089219331No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA60.2788104089219331No Hit
GGCACTAGAATGGACCTCTCCCTCATCACTACATTATTTCAATTAAATAC50.23234200743494424No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT50.23234200743494424No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT50.23234200743494424No Hit
AAATACCGTTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCAT50.23234200743494424No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA40.18587360594795538No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA40.18587360594795538No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCCTTGTTTTTAAGTAA40.18587360594795538No Hit
AAATACCGTTCGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT40.18587360594795538No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTAAGACGTGTAGAACCATGT40.18587360594795538No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT40.18587360594795538No Hit
AAATACCGTTAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT30.13940520446096655No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT30.13940520446096655No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT30.13940520446096655No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGACCC30.13940520446096655No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA30.13940520446096655No Hit
GGCACTAGAATGGACCTCTTTCTCATCACTACATTATTTCAATTAAATAC30.13940520446096655No Hit
GGCACTAGAATGGACCTCTCCTCATCACTACATTATTTCAATTAAATACA30.13940520446096655No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTACTTCAATTAAATAC30.13940520446096655No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA30.13940520446096655No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT30.13940520446096655No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)