FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151508

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151508
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3694
Total Bases535.3 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC234363.42717920952896No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG41011.099079588521928No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG1975.332972387655658No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA471.2723335138061722No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA270.7309149972929074No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA230.6226312939902545No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA230.6226312939902545No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC230.6226312939902545No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA220.5955603681645912No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG220.5955603681645912No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA200.5414185165132648No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA180.48727666486193827No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA180.48727666486193827No Hit
GGCACTAGAATGGACCTCTTTCTCATCACTACATTATTTCAATTAAATAC160.4331348132106118No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA150.4060638873849486No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA140.3789929615592853No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA120.32485110990795885No Hit
GGCACTAGAATGGACCTCTCCCTCATCACTACATTATTTCAATTAAATAC100.2707092582566324No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTAAGACGTGTAGAACCATGT90.24363833243096913No Hit
GGCACTAGAATGGACCTCTTCTCATCACTACATTATTTCAATTAAATACA80.2165674066053059No Hit
GGCACTAGAATGGCCTCTTCCTCATCACTACATTATTTCAATTAAATACA70.18949648077964265No Hit
AAATACGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT70.18949648077964265No Hit
GGCACTAGAATGGACCTCTCCTCATCACTACATTATTTCAATTAAATACA70.18949648077964265No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAAATAAATAC60.16242555495397942No Hit
AAATACCGTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT60.16242555495397942No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTACATTATTTCAATTAAATAC60.16242555495397942No Hit
GGCACTAGAATGGACCTCTTCCTCATAACTACATTATTTCAATTAAATAC60.16242555495397942No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT60.16242555495397942No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAA60.16242555495397942No Hit
GACACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC50.1353546291283162No Hit
GGCACTAGAATGGACCTCTTCCTCATCACCACATTATTTCAATTAAATAC50.1353546291283162No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAACAC50.1353546291283162No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTAAATTAAATAC50.1353546291283162No Hit
GGCACTAGAATGGACCTCTTCCCCATCACTACATTATTTCAATTAAATAC50.1353546291283162No Hit
AAATACCGTTCAGAACTGCCTTGTTTTAAGTAAGACGTGTAGAACCATGT50.1353546291283162No Hit
AAATACCGTTCAGACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT40.10828370330265295No Hit
AAATACCGTTAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT40.10828370330265295No Hit
GGCACTAGAATGGACCTCTTCCTCGTCACTACATTATTTCAATTAAATAC40.10828370330265295No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCCTTGTTTTTAAGTAA40.10828370330265295No Hit
GGCACTAGAATGGACCTCTTACTCATCACTACATTATTTCAATTAAATAC40.10828370330265295No Hit
GGCACTAGAATGGACCTCTTCCTCATCAATACATTATTTCAATTAAATAC40.10828370330265295No Hit
AAATACCGTTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCAT40.10828370330265295No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)