FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151529

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151529
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3172
Total Bases459.9 kbp
Sequences flagged as poor quality0
Sequence length139-145
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT186158.66960907944514No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTTCGTGAGACCAAGTCTC50215.825977301387137No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG1414.44514501891551No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTTTCGTGAGACCAAGTCTC1344.224464060529634No Hit
ACTTAAAAACAAGGCAGTGCTGTGCCCTCCCAGGTACCCCAAGCTGGCAG852.679697351828499No Hit
ACTTAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC581.8284993694829759No Hit
ACTTAAAAACAAGGCAGTGCTGAACGGTATTTTTCGTGAGACCAAGTCTC220.6935687263556116No Hit
ACTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC130.4098360655737705No Hit
ACTTAAAAACAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC120.37831021437578816No Hit
ACTTAAAAAAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC120.37831021437578816No Hit
ACTTAAAAACAAGGCAGCTCTGACGGTATTTTTCGTGAGACCAAGTCTCT110.3467843631778058No Hit
ACTTAAAAACAAGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC70.22068095838587645No Hit
ACTTAAAAACAAGGCAGTGCTATGCCCTCCCAGGTACCCCAAGCTGGCAG70.22068095838587645No Hit
CTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC60.18915510718789408No Hit
ACTTAAAACAAGGCAGCTCTGAACGGTATTTTTCGTGAGACCAAGTCTCT60.18915510718789408No Hit
ACTTAAAAACAAGGCAGTCTGAACGGTATTTTTCGTGAGACCAAGTCTCT60.18915510718789408No Hit
ACTTAAAAACAAGGCAGCTCTAACGGTATTTTTCGTGAGACCAAGTCTCT60.18915510718789408No Hit
ATTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC60.18915510718789408No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGTTACAGTTTCTGCCT50.15762925598991173No Hit
ACTTAAAAACAAGGCAGTGCTAATGACTAATGGTGCTACAGTTTCTGCCT50.15762925598991173No Hit
ACTTAAAAACAAGGCAGTGCTCAGGCCCTGCTCATGCTTCCCCAGACCCT50.15762925598991173No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTCGTGAGACCAAGTCTCT50.15762925598991173No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGCGCTACAGTTTCTGCCT50.15762925598991173No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTCTCTGCCT40.12610340479192939No Hit
ACTTAAAACAAGGCAGTGCTGTGCCCTCCCAGGTACCCCAAGCTGGCAGC40.12610340479192939No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCCGCCT40.12610340479192939No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCC40.12610340479192939No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAACGGTGCTACAGTTTCTGCCT40.12610340479192939No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)