FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151547

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151547
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences734
Total Bases106.4 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG24933.92370572207085No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT15821.525885558583106No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTTCGTGAGACCAAGTCTC14519.754768392370572No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTTTCGTGAGACCAAGTCTC628.446866485013624No Hit
ACTTAAAAACAAGGCAGTGCTGAACGGTATTTTTCGTGAGACCAAGTCTC121.6348773841961852No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTCGTGAGACCAAGTCTCT70.9536784741144414No Hit
ACTTAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC50.6811989100817438No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTTCGTGAGACCAAGTCTCT40.544959128065395No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTGAAG30.4087193460490463No Hit
ACTTAAAAACAAGGCAGCTCTGAACGTATTTTTCGTGAGACCAAGTCTCT30.4087193460490463No Hit
ACTTAAAACAAGGCAGCTCTGAACGGTATTTTTCGTGAGACCAAGTCTCT20.2724795640326975No Hit
ACTTAAAAACAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC20.2724795640326975No Hit
ACTTAAAACAAGGCAGTTCTGAACGGTATTTTTCGTGAGACCAAGTCTCT20.2724795640326975No Hit
ACTTAAAAACAAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTC20.2724795640326975No Hit
ACTTAAAAACAAGGCAGTGGTATCAACGAAGAGGTCCATTCTAGTGCCTT20.2724795640326975No Hit
TGTGTGTGTGTTTTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC20.2724795640326975No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCCGCCT20.2724795640326975No Hit
TGTGTGTGTGTTTCTGTGGCAGCTCTGAACGGTATTTTTCGTGAGACCAA20.2724795640326975No Hit
ACTAAAAACAAGGCATCTCTGAACGGTATTTTTCGTGAGACCAAGTCTCT10.13623978201634876No Hit
TGTGTGTGTGTTTCTGTGGTCCATTCTAGTGCCTTCGTGAGACCAAGTCT10.13623978201634876No Hit
ACTTAAAAACAAGGCAGTGCTGCCGGAGCAAAAGCGGTAGCGGGAGCCCG10.13623978201634876No Hit
TATGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG10.13623978201634876No Hit
ACTTAAAAACAAGGCAGCTCTGAACTATTTTTCGTGAGACCAAGTCTCTG10.13623978201634876No Hit
ACTTAAAAACAGGCAGTGCTAATGCCTAGTGGTGCTACAGTTTCTGCCTC10.13623978201634876No Hit
ACTTAAAAAAAGGCAGTTCTGAACGGTATTTTTCGTGAGACCAAGTCTCT10.13623978201634876No Hit
ACTTAAAAACAAGGCAGCTCTAAACGGTATTTTTCGTGAGACCAAGTCTC10.13623978201634876No Hit
ACTTAAAAACAAGGCAGCTTTGAACGGTATTTTTCGTGAGACCAAGTCTC10.13623978201634876No Hit
TGTGTGTGTGTTTATGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG10.13623978201634876No Hit
ACTTAAAAACAAGGCACTCTGAACGGTATTTTTCGTGAGACCAAGTCTCT10.13623978201634876No Hit
ACTTAAAAACAAGGCAGTCTGAACGGTATTTTTCGTGAGACCAAGTCTCT10.13623978201634876No Hit
ACTTAAAAACAAGGCAGTGCTAACGGTATTTTTCGTGAGACCAAGTCTCT10.13623978201634876No Hit
TGTGTGTGTGTTTCTGTGGTTTTCTTTACGGTTTGGACATAAGTTTAAAG10.13623978201634876No Hit
ACTTAAAAACAAGGCAGTTGTATCAACGAAGAGGTCCATTCTAGTGCCTT10.13623978201634876No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTACTACAGTTTCTGCCT10.13623978201634876No Hit
ACTTAAAAACAAGGCAGTTCTGACGGTATTTTTCGTGAGACCAAGTCTCT10.13623978201634876No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGTTACAGTTTCTGCCT10.13623978201634876No Hit
TGTGTGCGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG10.13623978201634876No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGAGAAGGGTAAAGCT10.13623978201634876No Hit
ACTTAAAAACAAGGCAGTGCTGAACGATATTTTTCGTGAGACCAAGTCTC10.13623978201634876No Hit
TTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC10.13623978201634876No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTCAAGGTTTGGACAGAAGGGTAAAG10.13623978201634876No Hit
TGTTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC10.13623978201634876No Hit
ACTTAAAAACAAGGCAGTGCTATCAATGAAGAGGTCCATTCTAGTGCCTT10.13623978201634876No Hit
ACTTAAAAACAAGGCAGTGGTATCAACGCAGAGGTCCATTCTAGTGCCTT10.13623978201634876Clontech Universal Primer Mix Long (95% over 22bp)
TGTGTGTTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAA10.13623978201634876No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGATAAAG10.13623978201634876No Hit
TGTGGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC10.13623978201634876No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTTTCGTGAGACGAAGTCTC10.13623978201634876No Hit
ATTAAAAACAAGGCAGCTCTGAACGGTATTTTTCGTGAGACCAAGTCTCT10.13623978201634876No Hit
TGTGTGTGTGTTTCTGTGGTTTTCTTTAAGGTTTGGACAGAAGGGTAAAG10.13623978201634876No Hit
ACTTAAAACAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTCT10.13623978201634876No Hit
TGTGTTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC10.13623978201634876No Hit
CTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC10.13623978201634876No Hit
TACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCC10.13623978201634876No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGGAGGGTAAAG10.13623978201634876No Hit
ACTTAAAAACAAGGGAGCTCTGAACGGTATTTTTCGTGAGACCAAGTCTC10.13623978201634876No Hit
ACTTAAAAACAAGGCAGCTCTAACGGTATTTTTCGTGAGACCAAGTCTCT10.13623978201634876No Hit
ACTTAAAAACAAGGCAGTACTAATGCCTAATGGTGCTACAGTTTCTGCCT10.13623978201634876No Hit
TGTGTGTGTGTTTTTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG10.13623978201634876No Hit
ATTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC10.13623978201634876No Hit
TGTGTGTGTGTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC10.13623978201634876No Hit
ACTTAAAAACAAGGAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC10.13623978201634876No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAGGGGTAAAG10.13623978201634876No Hit
ACTTAAAAACAAAGCAGTGGTATCAACGCAGAGGTCCATTCTAGTGCCTT10.13623978201634876Clontech Universal Primer Mix Long (95% over 22bp)
TGTGTGTGTGTTTCTGTGGGTGTCTTTAAGGTTTGGACAGAAGGGTAAAG10.13623978201634876No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAAGTTTGGACAGAAGGGTAAAG10.13623978201634876No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACGGAAGGGTAAAG10.13623978201634876No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTTCGAGAGACCAAGTCTC10.13623978201634876No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTGCAGTTTCTGCCT10.13623978201634876No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTGAGGTTTGGACAGAAGGGTAAAG10.13623978201634876No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAGTGGTGCTACAGTTTCTGCCT10.13623978201634876No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTATAG10.13623978201634876No Hit
TCTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG10.13623978201634876No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTCTAAGGTTTGGACAGAAGGGTAAAG10.13623978201634876No Hit
ACTTAAAAACAAGGCAGCTCTGACGGTATTTTTCGTGAGACCAAGTCTCT10.13623978201634876No Hit
ACTTAAAAACAAGTAATCTCAGAACAGTAATTTTCGTGAGACCAAGACTA10.13623978201634876No Hit
ACTTAAAAACAAGGCGGCTCTGAACGGTATTTTTCGTGAGACCAAGTCTC10.13623978201634876No Hit
ACTTAAAAAAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC10.13623978201634876No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTTGACAGAAGGGTAAAG10.13623978201634876No Hit
TGTGTGTGTGTTTCTGCGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG10.13623978201634876No Hit
ACTTAAAAACAAGGCAATGCTAATGCCTAATGGTGCTACAGTTTCTGCCT10.13623978201634876No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGATTTTCGTGAGACCAAGTCTCTG10.13623978201634876No Hit
ACTAAAAACAAGGCAGCTCTGAACGGTATTTTTCGTGAGACCAAGTCTCT10.13623978201634876No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGATTTTTCGTGAGACCAAGTCTCT10.13623978201634876No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCAAATGGTGCTGCAGTTTCTGCCT10.13623978201634876No Hit
TGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGCT10.13623978201634876No Hit
TGTGTGTGTGTTTCAGTGGGTATCAACGCAGAGTACATGGAAGCAGTGGT10.13623978201634876No Hit
ACTTAAAAACAAGGCAGTGCTAAGGCCTAATGGTGCTACAGTTTCTGCCT10.13623978201634876No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)