FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151551

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151551
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences753
Total Bases109.1 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT27035.85657370517929No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG24432.40371845949535No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTTCGTGAGACCAAGTCTC9112.084993359893758No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTTTCGTGAGACCAAGTCTC435.710491367861885No Hit
ACTTAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC121.593625498007968No Hit
ACTTAAAAACAAGGCAGTGCTGAACGGTATTTTTCGTGAGACCAAGTCTC50.6640106241699867No Hit
ACTTAAAAACAAGGCAGTGCTATCAACGAAGAGGTCCATTCTAGTGCCTT40.5312084993359893No Hit
ACTTAAAACAAGGCAGCTCTGAACGGTATTTTTCGTGAGACCAAGTCTCT30.398406374501992No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTCGTGAGACCAAGTCTCT20.2656042496679947No Hit
ACTTAAAAACAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC20.2656042496679947No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGATTTTTCGTGAGACCAAGTCTCT20.2656042496679947No Hit
ACTTAAAACAAGGCAGTTCTGAACGGTATTTTTCGTGAGACCAAGTCTCT20.2656042496679947No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCAT20.2656042496679947No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGTCT20.2656042496679947No Hit
ACTTAAAAACAAGGCAGTGCTAATGTCTAATGGTGCTACAGTTTCTGCCT20.2656042496679947No Hit
TGTGTGTGTGTTTCTGTAGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG20.2656042496679947No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCC20.2656042496679947No Hit
TGTGTGTGTTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC20.2656042496679947No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAGGGTTTGGACAGAAGGGTAAAG20.2656042496679947No Hit
ACTTAAAAAAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC20.2656042496679947No Hit
TGTGTGTGTATTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG10.13280212483399734No Hit
ACTTAAAAACAAGGCAGTGCTAACGCCTAATGGTGCTACAGTTTCTGCCT10.13280212483399734No Hit
TGTGTGTGTGTTTCTTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC10.13280212483399734No Hit
ACTTAAAAACAAGGCAGCTCGAACGGTATTTTTCGTGAGACCAAGTCTCT10.13280212483399734No Hit
ACTTAAAAACAAGGCAGTGCTGCGAGGAGTGGGTCTGTGACGAGGATAGT10.13280212483399734No Hit
ACTTAAAAACAAGGCAGCTCTGAAGGTATTTTTCGTGAGACCAAGTCTCT10.13280212483399734No Hit
ACTTAAAAACAAGGCAGTGCTGATGCCTAATGGTGCTACAGTTTCTGCCT10.13280212483399734No Hit
TGTGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAA10.13280212483399734No Hit
ATTAAAAACAAGGCAGTTCTGAACGGTATTTTTCGTGAGACCAAGTCTCT10.13280212483399734No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCGGCCT10.13280212483399734No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCCACAGTTTCTGCCT10.13280212483399734No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGATACAGTTTCTGCCT10.13280212483399734No Hit
ACTTAAAAACAAGGCAGTGCTATCAACGAAGAGGTCATTCTAGTGCCTTC10.13280212483399734No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGAAAGAAGTGTAAAG10.13280212483399734No Hit
ACTTAAAAACAAGGCAGCCCTGAACGGTATTTTTCGTGAGACCAAGTCTC10.13280212483399734No Hit
ACTTAAAAACAAGGCAGTGCTGAACGGTATTTTTCGTGATCCCAAGTCTC10.13280212483399734No Hit
ACTTAAAAAACAAGGCAGTGCTATCAACGAAGAGGTCCATTCTAGTGCCT10.13280212483399734No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTCCTGCCT10.13280212483399734No Hit
ACTTAAAAACAAGGAAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT10.13280212483399734No Hit
ACTTAAAAACAAGGCAGTTCTACGGTATTTTCGTGAGACCAAGTCTCTGC10.13280212483399734No Hit
ACTTAAAAACAAGGCAGTGGTATTAACGAAGAGGTCCATTCTAGTGCCTT10.13280212483399734No Hit
CCTTAAAAACAAGGCAGTTCTAATGCCTAATGGTGCTACAGTTTCTGCCT10.13280212483399734No Hit
ACTTAAAAACAAGGCAGTGGTATCAACGCAGAGGTCCATTCTAGTGCCTT10.13280212483399734Clontech Universal Primer Mix Long (95% over 22bp)
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAACGGTAAAG10.13280212483399734No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTTAGTGAGACCAACTCTC10.13280212483399734No Hit
ACTTAAAAACAAGGCAGTTCTGAACGTATTTTCGTGAGACCAAGTCTCTG10.13280212483399734No Hit
TGTGTGTGTGTTTCTGTGGATTTCTTTAAGGTTTGGACAGAAGGGTAAAG10.13280212483399734No Hit
ACTTAAAAACAAGGCAGTTCTGAAACGGTATTTTTCGTGAGACCAAGTCT10.13280212483399734No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGATTGGACAGAAGGGTAAAG10.13280212483399734No Hit
TGTTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAA10.13280212483399734No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGAGAGAAGGGTAAAG10.13280212483399734No Hit
ACTTAAAAACAAGGCAGTGCTAATGACTAATGGTGCTACAGTTTCTGCCT10.13280212483399734No Hit
ACTTAAAAACAAGGCAGTTCTAATGCCTAATGGTGCTACAGTTTCTGCCT10.13280212483399734No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTTCGTGAGACCAAGTCTCT10.13280212483399734No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTCTTCGTGAGACAAAGTCTC10.13280212483399734No Hit
ACTTAAAAACAAGGCAGTGCTAGTGCCTAATGGTGCTACAGTTCCTGCCT10.13280212483399734No Hit
ACTTAAAAACAAGGCAGTGCTATCAACGAAGAGGTCCATTTAGTGCCTTC10.13280212483399734No Hit
ACTTAAAAACAAGGAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC10.13280212483399734No Hit
ACTTAAAACAAGGCAGTGCTGAACGGTATTTTTCGTGAGACCAAGTCTCT10.13280212483399734No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAGGGGTAAAG10.13280212483399734No Hit
ACTTAAAAACAAGGCAGTGATAATGCCTAATGGTGCTACAGTTTCTGCCT10.13280212483399734No Hit
TGGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC10.13280212483399734No Hit
ACTTAAAAACAAGGCAGCTCTGAAACGGTATTTTTCGTGAGACCAAGTCT10.13280212483399734No Hit
TGTGTGTGTGGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAA10.13280212483399734No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGATAGAAGGGTAAAG10.13280212483399734No Hit
ACTTAAAAACAAGGCGGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT10.13280212483399734No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAGTGGTGCTACAGTTTCTGCCT10.13280212483399734No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTCTAAGGTTTGGACAGAAGGGTAAAG10.13280212483399734No Hit
ACTTAAAAACAAGGCAGTGCTGAACGGTATTTTCGTGAGACCAAGTCTCT10.13280212483399734No Hit
TGTGTGTGTGTTTCTGTGGCAGCTCTGAACGGTATTTTTCGTGAGACCAA10.13280212483399734No Hit
ACTTAAAAAAAGGCAGTGCTGAACGGTATTTTTCGTGAGACCAAGTCTCT10.13280212483399734No Hit
ACTTAAAAACAAGGCAATGCTAATGCCTAATGGTGCTACAGTTTCTGCCT10.13280212483399734No Hit
ACTTAAAAACAAGGCATCTCTGAACGGTATTTTTCGTGAGACCAAGTCTC10.13280212483399734No Hit
ACTTAAAGACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT10.13280212483399734No Hit
GTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC10.13280212483399734No Hit
AGTTAAAAACAAGGCAGCTCTGAACGGTATTTTTCGTGAGACCAAGTCTC10.13280212483399734No Hit
ATTAAAAACAAGGCAGTGCTCAGGCCCTGCTCATGCTTCCCCAGACCCTG10.13280212483399734No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)