FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151561

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151561
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6387
Total Bases926 kbp
Sequences flagged as poor quality0
Sequence length141-145
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT350154.81446688586191No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTTCGTGAGACCAAGTCTC111317.426021606387977No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTTTCGTGAGACCAAGTCTC3425.3546265852512915No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG1772.7712541099107564No Hit
ACTTAAAAACAAGGCAGTGCTGTGCCCTCCCAGGTACCCCAAGCTGGCAG1682.6303428839830905No Hit
ACTTAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC961.5030530765617662No Hit
ACTTAAAAACAAGGCAGTGCTGAACGGTATTTTTCGTGAGACCAAGTCTC641.0020353843745107No Hit
ACTTAAAAACAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC380.5949585094723657No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTCGTGAGACCAAGTCTCT330.5166744950681071No Hit
ACTTAAAACAAGGCAGCTCTGAACGGTATTTTTCGTGAGACCAAGTCTCT260.40707687490214495No Hit
ACTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC260.40707687490214495No Hit
ACTTAAAAACAAGGCAGTGCTATGCCCTCCCAGGTACCCCAAGCTGGCAG250.39142007202129325No Hit
ACTTAAAAACAAGGCAGTGCTATCAACGAAGAGGTCCATTCTAGTGCCTT240.37576326914044156No Hit
ACTTAAAAAAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC160.25050884609362767No Hit
ATTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC160.25050884609362767No Hit
ACTTAAAAACAAGGCAGCTCTGAACGTATTTTTCGTGAGACCAAGTCTCT150.23485204321277595No Hit
ACTTAAAAACAAGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC140.21919524033192422No Hit
ACTTAAAAACAAGGCAGTGTTAATGCCTAATGGTGCTACAGTTTCTGCCT120.18788163457022078No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTTCGTGAGACCAAGTCTCT110.17222483168936903No Hit
ACTTAAAAACAAGGCAGTGGTATCAACGAAGAGGTCCATTCTAGTGCCTT110.17222483168936903No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCC110.17222483168936903No Hit
ACTTAAAAACAAGGCAGCTCTGACGGTATTTTTCGTGAGACCAAGTCTCT100.1565680288085173No Hit
ACTTAAAACAAGGCAGTTCTGAACGGTATTTTTCGTGAGACCAAGTCTCT100.1565680288085173No Hit
ACTTAAAAACAAGGAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC100.1565680288085173No Hit
ACTTAAAAACAAGGCAGTGCTAATGACTAATGGTGCTACAGTTTCTGCCT90.14091122592766556No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTCCTGCCT90.14091122592766556No Hit
ACTTAAAAACAAGGCAGTTCTAATGCCTAATGGTGCTACAGTTTCTGCCT90.14091122592766556No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCCAATGGTGCTACAGTTTCTGCCT80.12525442304681383No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCCACAGTTTCTGCCT70.10959762016596211No Hit
ACTTAAAAACAAGGCAGTGCTAACGCCTAATGGTGCTACAGTTTCTGCCT70.10959762016596211No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGTCT70.10959762016596211No Hit
ACTTAAAAACAAGGCAGCTCTAACGGTATTTTTCGTGAGACCAAGTCTCT70.10959762016596211No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAACGGTGCTACAGTTTCTGCCT70.10959762016596211No Hit
ACTTAAAAACAAGGCAGCTCTGAAACGGTATTTTTCGTGAGACCAAGTCT70.10959762016596211No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTGCAGTTTCTGCCT70.10959762016596211No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)