FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151564

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151564
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1128
Total Bases163.5 kbp
Sequences flagged as poor quality0
Sequence length144-145
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC29325.975177304964536No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC22820.212765957446805No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG18015.957446808510639No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC13011.52482269503546No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG524.609929078014184No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT110.975177304964539No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG90.7978723404255319No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA80.7092198581560284No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA70.6205673758865249No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT70.6205673758865249No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA60.5319148936170213No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA40.3546099290780142No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT40.3546099290780142No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAA40.3546099290780142No Hit
AAATACCGTTCGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT30.26595744680851063No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA30.26595744680851063No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA30.26595744680851063No Hit
AAATACCGTTCAGACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT30.26595744680851063No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA30.26595744680851063No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTAAGACGTGTAGAACCATGT30.26595744680851063No Hit
AAATACCGTTCAAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT30.26595744680851063No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT30.26595744680851063No Hit
AAATACCGTTCAGAGTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT20.1773049645390071No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTACAAATTGCTGCTGGAACC20.1773049645390071No Hit
AAATACCGTTCAGAGCTGCCTGTTTTTAAGTAAGACGTGTAGAACCATGT20.1773049645390071No Hit
GGCACTAGAATGGACCTCTTTCTCATCACTACATTATTTCAATTAAATAC20.1773049645390071No Hit
GGCACTAGATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAAG20.1773049645390071No Hit
AAATACCGTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT20.1773049645390071No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT20.1773049645390071No Hit
AAATACCGTTCAGAGTTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG20.1773049645390071No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA20.1773049645390071No Hit
AAATACCGTTAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT20.1773049645390071No Hit
AAATACCGTTCAGAGCTGCCACCAGGATCTTGCAAATTGCTGCTGGAACC20.1773049645390071No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA20.1773049645390071No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTACTGCTGGAACC20.1773049645390071No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCCTTGTTTTTAAGTAA20.1773049645390071No Hit
AAATACCGTTCAGAACTGCCTTGTTTTAAGTAAGACGTGTAGAACCATGT20.1773049645390071No Hit
GGCACTAGAATGGACCTCTTCTCATCACTACATTATTTCAATTAAATACA20.1773049645390071No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)