FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151579

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151579
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences720
Total Bases104.3 kbp
Sequences flagged as poor quality0
Sequence length144-145
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT24934.583333333333336No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG15521.52777777777778No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTTCGTGAGACCAAGTCTC13919.305555555555557No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTTTCGTGAGACCAAGTCTC628.61111111111111No Hit
ACTTAAAAACAAGGCAGTGCTGAACGGTATTTTTCGTGAGACCAAGTCTC81.1111111111111112No Hit
ACTTAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC60.8333333333333334No Hit
ACTTAAAAACAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC40.5555555555555556No Hit
ACTTAAAACAAGGCAGCTCTGAACGGTATTTTTCGTGAGACCAAGTCTCT40.5555555555555556No Hit
ACTTAAAAACAAGGCAGTGCTATCAACGAAGAGGTCCATTCTAGTGCCTT40.5555555555555556No Hit
ACTTAAAACAAGGCAGTTCTGAACGGTATTTTTCGTGAGACCAAGTCTCT30.4166666666666667No Hit
ACTTAAAAACAAGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC30.4166666666666667No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTCGTGAGACCAAGTCTCT20.2777777777777778No Hit
ACTTAAAAACAAGGCAGCTCTGAAGGTATTTTTCGTGAGACCAAGTCTCT20.2777777777777778No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCCACAGTTTCTGCCT20.2777777777777778No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTACCT20.2777777777777778No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAGGGGTAAAG20.2777777777777778No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCC20.2777777777777778No Hit
ACTTAAAAAAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC20.2777777777777778No Hit
CTTAAAAACAAGGCAGTTCTGAACGGTATTTTTCGTGAGACCAAGTCTCT10.1388888888888889No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGGTACAGTTTCTGCCT10.1388888888888889No Hit
ACTTAAAAACAAGGCAGTTCTAACGGTATTTTTCGTGAGACCAAGTCTCT10.1388888888888889No Hit
ACTTAAAACAAGGCAGTGCTAATACCTAATGGTGCTACAGTTTCTGCCTC10.1388888888888889No Hit
ACTTAAAAACAAGGCAGTGTAATGCCTAATGGTGCTACAGTTTCTGCCTC10.1388888888888889No Hit
ACTAAAAACAAGGCAGTGCTAATGCCTAGTGGTGCTACAGTTTCTGCCTC10.1388888888888889No Hit
TGTGTGTGTGTTTCTTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC10.1388888888888889No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTAAGGTTTGGATAGAAGGGTAAAGC10.1388888888888889No Hit
ACTTAAAAAAAGGCAGTTCTGAACGGTATTTTTCGTGAGACCAAGTCTCT10.1388888888888889No Hit
ACTTAAAAACAAGGCAGTGCTGATGCCTAATGGTGCTACAGTTTCTGCCT10.1388888888888889No Hit
ACTTAAAAACAAGGCAGCCTGAACGGTATTTTCGTGAGACCAAGTCTCTG10.1388888888888889No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTTCGTGAGACCAACTCTC10.1388888888888889No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGTTGCTACAGTTTCTGCCT10.1388888888888889No Hit
ACTTAAAAACAAGGCAGTCTGAACGGTATTTTTCGTGAGACCAAGTCTCT10.1388888888888889No Hit
ACGTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT10.1388888888888889No Hit
ACTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC10.1388888888888889No Hit
ACTTAAAACAAGGCGGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC10.1388888888888889No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGATACAGTTTCTGCCT10.1388888888888889No Hit
TGTGTGTGTGTTTCTGTGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC10.1388888888888889No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAAAAGGGTAAAG10.1388888888888889No Hit
ACTTAAGAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT10.1388888888888889No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGTTACAGTTTCTGCCT10.1388888888888889No Hit
ACTTAAAAACAAGGCAGTTCTGACGGTATTTTTCGTGAGACCAAGTCTCT10.1388888888888889No Hit
TTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC10.1388888888888889No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTCAAGGTTTGGACAGAAGGGTAAAG10.1388888888888889No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGAGCTACAGTTTCTGCCT10.1388888888888889No Hit
TGTTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC10.1388888888888889No Hit
ACTTAAAAACAAGGCAGTGGTATCAACGCAGAGGTCCATTCTAGTGCCTT10.1388888888888889Clontech Universal Primer Mix Long (95% over 22bp)
ACTTAAAAACATGGCAGCTCTGAACGGTATTTTTCGTGAGACCAAGTCTC10.1388888888888889No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGTCT10.1388888888888889No Hit
ACTTAAAAACAGGCAGTACTAATGCCTAATGGTGCTACAGTTTCTGCCTC10.1388888888888889No Hit
ACTTAAAAACAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCCGCCTC10.1388888888888889No Hit
ACTTAAAAACAAGGCAGTTCTGAAACGGTATTTTTCGTGAGACCAAGTCT10.1388888888888889No Hit
TGTGTGTGTGTTTCTGTGGCAGTTCTGAACGGTATTTTTCGTGAGACCAA10.1388888888888889No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAA10.1388888888888889No Hit
ACTTAAAACAGGCAGCTCTGAACGGTATTTTTCGTGAGACCAAGTCTCTG10.1388888888888889No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCAAATGGTGCTACAGTTTCTGCCT10.1388888888888889No Hit
CTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC10.1388888888888889No Hit
ACTTAAAAACAAGGCAGTGCTGACGGTATTTTTCGTGAGACCAAGTCTCT10.1388888888888889No Hit
ACTTAAAAACAAGGCAGTGCTAATGACTAATGGTGCTACAGTTTCTGCCT10.1388888888888889No Hit
AGTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT10.1388888888888889No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCCGCCT10.1388888888888889No Hit
ACTTAAAAACAAGGCAGCTCTAACGGTATTTTTCGTGAGACCAAGTCTCT10.1388888888888889No Hit
ACTTAAAAACAAGGCAGTGTTAATGCCTAATGGTGCTACAGTTTCTGCCT10.1388888888888889No Hit
ACTTAAAAACAAGGCAGTGCTAATGTCTAATGGTGCTACAGTTTCTGCCT10.1388888888888889No Hit
ACTTAAAAACAAGGCAGTGCCAATGCCTAATGGTGCTACAGTTTCTGCCT10.1388888888888889No Hit
TGTGTGTGTGTTTCTGCCCCTTCCGTGGAACACACACATGGTGAACTCCT10.1388888888888889No Hit
ACTTAAAAACAAGGCAGTTCTGAACGTATTTTTCGTGAGACCAAGTCTCT10.1388888888888889No Hit
ATTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC10.1388888888888889No Hit
ACTTAAAAACAAGGCAGTGCTAATGCATAATGGTGCTACAGTTTCTGCCT10.1388888888888889No Hit
TGTGTGTGTGTTCCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG10.1388888888888889No Hit
ACTTTAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT10.1388888888888889No Hit
ACTTAAAAACAAGCCAGCTCTGAACGGTATTTTTCGTGAGAACAAGTCTC10.1388888888888889No Hit
ACTTAAAAACAAGGCAGCTCTGAAACGGTATTTTTCGTGAGACCAAGTCT10.1388888888888889No Hit
ACTTAAAAACAAGGCAGTGCTATCAACGAAGAGGTCCATTCTAGTCCTTC10.1388888888888889No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGCGCTACAGTTTCTGCCT10.1388888888888889No Hit
TGTGTGTGTTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC10.1388888888888889No Hit
ACTTAAAAATAAGGCAGCTCTGAACGGTATTTTTCGTGAGACCAAGTCTC10.1388888888888889No Hit
ACTTAAAAACAAGGCATGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC10.1388888888888889No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAGTGGTGCTACAGTTTCTGCCT10.1388888888888889No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATAGTGCTACAGTTTCTGCCT10.1388888888888889No Hit
TCTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG10.1388888888888889No Hit
ACTTAAAAACAAGGCAGCTCTGACGGTATTTTTCGTGAGACCAAGTCTCT10.1388888888888889No Hit
TGTGTGTGTGTTTCTGTGGCAGCTCTGAACGGTATTTTTCGTGAGACCAA10.1388888888888889No Hit
TGTGTGTGCGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG10.1388888888888889No Hit
TGTGTGTGTGTTTCTGGGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG10.1388888888888889No Hit
ACTTAAAAACAAGGCAGTGTTAATGCCTAATGGTGATACAGTTTCTGCCT10.1388888888888889No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTTTGCCT10.1388888888888889No Hit
GTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC10.1388888888888889No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)