FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151601

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151601
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences803
Total Bases116.4 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTTCGTGAGACCAAGTCTC45656.78704856787049No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTTTCGTGAGACCAAGTCTC14217.68368617683686No Hit
ACTTAAAAACAAGGCAGTGCTGAACGGTATTTTTCGTGAGACCAAGTCTC293.61145703611457No Hit
ACTTAAAAACAAGGCAGTGCTATCAACGAAGAGGTCCATTCTAGTGCCTT232.86425902864259No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTCGTGAGACCAAGTCTCT131.61892901618929No Hit
ACTTAAAAACAAGGCAGCTCTGAACGTATTTTTCGTGAGACCAAGTCTCT101.2453300124533No Hit
ACTTAAAAACAAGGCAGTGGTATCAACGAAGAGGTCCATTCTAGTGCCTT101.2453300124533No Hit
ACTTAAAACAAGGCAGCTCTGAACGGTATTTTTCGTGAGACCAAGTCTCT70.8717310087173101No Hit
ACTTAAAACAAGGCAGTTCTGAACGGTATTTTTCGTGAGACCAAGTCTCT60.7471980074719801No Hit
ACTTAAAAACAAGGCAGTCTGAACGGTATTTTTCGTGAGACCAAGTCTCT50.62266500622665No Hit
ACTTAAAAACAAGGCAGCTCTAACGGTATTTTTCGTGAGACCAAGTCTCT40.49813200498132004No Hit
ACTTAAAAACAAGGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGT40.49813200498132004Clontech SMARTer II A Oligonucleotide (96% over 25bp)
ACTTAAAAACAAGGCAGTGCTATCAACGCAGAGTACATGGAAGCAGTGGT40.49813200498132004No Hit
ACTTAAAAACAAGGCAGCTCTGAAGGTATTTTTCGTGAGACCAAGTCTCT20.24906600249066002No Hit
ACTTAAAAACAAAGCAGTGGTATCAACGAAGAGGTCCATTCTAGTGCCTT20.24906600249066002Clontech Universal Primer Mix Long (95% over 22bp)
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTTCTTGAGACCAAGTCTC20.24906600249066002No Hit
ATTAAAAACAAGGCAGCTCTGAACGGTATTTTTCGTGAGACCAAGTCTCT20.24906600249066002No Hit
ACTTAAAAACAAGGCAGTGCTGACGGTATTTTTCGTGAGACCAAGTCTCT20.24906600249066002No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTTCGTGAGACCAAGTCTCT20.24906600249066002No Hit
ACTTAAAACAAGGCAGTGCTGAACGGTATTTTTCGTGAGACCAAGTCTCT20.24906600249066002No Hit
TGTGTGTGTGTTTCTGTGGCAGCTCTGAACGGTATTTTTCGTGAGACCAA20.24906600249066002No Hit
ACTTAAAAACAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA20.24906600249066002Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTAAAAACAAGGCAGTTCTGAACGGTATTTTTCGTGAGACCAAGTCTCT10.12453300124533001No Hit
ACTTAAAAACAAGGCAGTGCTATCAACGAAGAGGTCCATTCAGTGCCTTC10.12453300124533001No Hit
ACTTAAAAACAAGGCAGTTATGAACGGTATTTTTCGTGAGACAAACTCTC10.12453300124533001No Hit
ACTTAAAAAAAGGCAGCTCTGAACGGTATTTTTCGTGAGACCAAGTCTCT10.12453300124533001No Hit
ACTTAAAAACAAGGCAGTGCTATCAACACAGAGTACATGGAAGCAGTGGT10.12453300124533001No Hit
ACTTAAAAACAACGCAGCTCTGAACGGTATTTTTCGTGAGACCAAGTCTC10.12453300124533001No Hit
ACTTAAAAACAAGGCAGTGCTCTGAACGGTATTTTTCGTGAGACCAAGTC10.12453300124533001No Hit
ACTTAAAAACAAGGCAGTGCTATCAACGCAGAGGTCCATTCTAGTGCCTT10.12453300124533001No Hit
ACTTAAAAGCAAGGCAGCTCTGAACGGTATTTTTCGTGAGACCAAGTCTC10.12453300124533001No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGATTTTTCGTGAGACCAAGTCTCT10.12453300124533001No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTGTTTTTCGTGAGACCAAGTCTC10.12453300124533001No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTTCGTGAGACCAACTCTC10.12453300124533001No Hit
ATTAAAAACAAGGCAGTGCTATCAACGAAGAGGTCCATTCTAGTGCCTTC10.12453300124533001No Hit
ACTAAAACAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC10.12453300124533001Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTTAAAAACAATGCAGCTCTGAACGGTATTTTTCGTGAGACCAAGTCTC10.12453300124533001No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTTCGTGAGACCCAGTCTC10.12453300124533001No Hit
ACTTAAAAACAAGACAGCTCTGAACGGTATTTTTCGTGAGACCAAGTCTC10.12453300124533001No Hit
ACTTAAAAACAAGGCAGTGTACATGGAAGCAGTGGTATCAACGCAGAGTA10.12453300124533001Clontech Universal Primer Mix Long (96% over 25bp)
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTTCGTGAGCCCAAGTCTC10.12453300124533001No Hit
ACTTAAAAACAAGGCAGCTCTGAACGATTTTTCGTGAGACCAAGTCTCTG10.12453300124533001No Hit
TGTGTGTGTGTTTCTGTGGGCTCTGAACGGTATTTTTCGTGAGACCAAGT10.12453300124533001No Hit
ACTTAAAAACAAGGCAGTGCTGAACGGTATTTTTCGTGGGACCAAGTCTC10.12453300124533001No Hit
ACTTAAAAACAAGGCAGCTCTGATCGGTATTTTTCGTGAGACCACGTCTC10.12453300124533001No Hit
ACTTAAAACAAGGCAGTGCTATCAACGAAGAGGTCCATTCTATGCCTTCG10.12453300124533001No Hit
ACTTAAAAACAAGGCAGTTCCGAACGGTATTTTTCGTGAGACCAAGTCTC10.12453300124533001No Hit
ACTTAAAACAAGGCAGCTATGAACGGTATTTTTCGTGAGACCAAGTCTCT10.12453300124533001No Hit
TGTGTGTGTGTTTCTGTGGGTAAGCAGTGGTATCAACGCAGAGGTCCATT10.12453300124533001Clontech Universal Primer Mix Long (96% over 26bp)
ACTTAAAAACAAGGGAGTTCTGAACGGTATTTTTCGTGAGACCAAGTCTC10.12453300124533001No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATATTTCGTGAGACCAAGTCTC10.12453300124533001No Hit
TCTTAAAAACAAGGCAGCTCTGAACGGTATTTTTCGTGAGACCAAGTCTC10.12453300124533001No Hit
ACTTAAAAACAAGGCAGTGCTTCAACGCAGAGGTCCATTCTAGTGCCTTC10.12453300124533001No Hit
ACGTAAAAACAAGGCAGCTCTGAACGGTATTTTTCGTGAGACCAAGTCTC10.12453300124533001No Hit
ACTTAAAAACAAGGCAGTGCTACCGTAGGCAGCTCTGAACGGTATTTTTC10.12453300124533001No Hit
ACTTAAAAACAAGGCAGCTCTGAACCGTATTTTTCGTGAGACCAACACTC10.12453300124533001No Hit
ACTTAAAAACAAGGCAGTGCTATCAACGCAGAGTACATGGGAAGCAGTGG10.12453300124533001No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTTCGTGAGACCAAGACTC10.12453300124533001No Hit
TGTGTGTGTGCTTCTGTGGCAGCTCTGAACGGTATTTTTCGTGAGACCAA10.12453300124533001No Hit
ACTTAAAAAAGGCAGTGCTATCAACGAAGAGGTCCATTCTAGTGCCTTCG10.12453300124533001No Hit
ACTTAAAAACAAGGCAGTGCTATCAACGAAGAGGCCATTCTAGTGCCTTC10.12453300124533001No Hit
ACTTAAAAACAGGCAGCTCTGAACGGTATTTTTCGTGAGACCAAGTCTCT10.12453300124533001No Hit
ACTTAAAAACAAGGCAGCTCAGAACGGTATTTTTCGTGAGACCAAGTCTC10.12453300124533001No Hit
ACTTAAAAACAAGGCAGTGCTATCAACGAAGGGTCCATTCTAGTGCCTTC10.12453300124533001No Hit
GCTTAAAAACAAGGCAGTTCTGAACGGTATTTTTCGTGAGACCAAGTCTC10.12453300124533001No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTGTTTTTCGTGAGACCAAGTCTC10.12453300124533001No Hit
ACTTAAAAACAAGCAGTGGTATCAACGCAGAGTACATGGGATCAGTGGTA10.12453300124533001Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTTAAAAACAAGGCAGTTCTGAACGTATTTTTCGTGAGACCAAGTCTCT10.12453300124533001No Hit
ACTTAAAGACAAGGCAGCTCTGAACGGTATTTTTCGTGAGACCAAGTCTC10.12453300124533001No Hit
ACTTAAAAACAAGGCAGTTCTAAACGGTATTTTTCGTGAGACCAAGTCTC10.12453300124533001No Hit
ACTTAAAAACAAGGCAGCTCGAACGGTATTTTCGTGAGACCAAGTCTCTG10.12453300124533001No Hit
ACTTAAAAACAAGGCAGATCTGAACGGTATTTTTCGTTAGACCAAGTCTC10.12453300124533001No Hit
ACTTAAAAACAAAGCAGTGGTATCAACGCAGAGGTCCATTCTAGTGCCTT10.12453300124533001Clontech Universal Primer Mix Long (95% over 22bp)
ACTTAAAAACAAGGCAGTTCTGAACTATTTTTCGTGAGACCAAGTCTCTG10.12453300124533001No Hit
ACTTAAACAAGGCAGTTCTGAACGGTATTTTTCGTGAGACCAAGTCTCTG10.12453300124533001No Hit
ACCTTAAAAACAAGGCAGCTCTGAACGGTATTTTTCGTGAGACCAAGTCT10.12453300124533001No Hit
ACTTAAAAACAAGGCAGTGCTATCAACGAAGAGGTCCATTCTTGTGCCTT10.12453300124533001No Hit
ACTTAAAAACAAGGCAGCTCTGAAACGGTATTTTTCGTGAGACCAAGTCT10.12453300124533001No Hit
TGTGTGTGTGTTTCTGTGGGTAAGCAGTGGTATCAACGAAGAGGTCCATT10.12453300124533001Clontech SMARTer II A Oligonucleotide (95% over 22bp)
ACTTAAAAACAAAGCAGTGGTATCAACGAAGAGGTCCATTTAGTGCCTTC10.12453300124533001Clontech Universal Primer Mix Long (95% over 22bp)
ACTTAAAAACAAGGCAGTGCTATCAACGAAGAGGTCCATTCTAGTCCTTC10.12453300124533001No Hit
ACTTTAAAACAAGGCAGTTCTGAACGGTATTTTTCGTGAGACCAAGTCTC10.12453300124533001No Hit
ACTTAAAAATAAGGCAGTTCTGAACGGTATTTTTCGTGAGACCAAGTCTC10.12453300124533001No Hit
ACTTAAAAACAAGGCAGTGCTATCAACGAAGAGGTCCATTCTAGTGCTTC10.12453300124533001No Hit
ACTTAAAAATAAGGCAGCTCTGAACGGTATTTTTCGTGAGACCAAGTCTC10.12453300124533001No Hit
ACTTAAAGACAAGGCAGTGCTATCAACGAAGAGGTCCATTCTAGTGCCTT10.12453300124533001No Hit
TGTGTGTGTGTTTCTGTGGCAGCTCTGAACGGTATTTTCGTGAGACCAAG10.12453300124533001No Hit
ACTTAAAAACAAGGCAGCTCTGACGGTATTTTTCGTGAGACCAAGTCTCT10.12453300124533001No Hit
ACTTAAAAACAAGGCAGTGCTAAACGGTATTTTTCGTGAGACCAAGTCTC10.12453300124533001No Hit
ACTAAAAACAAGGCAGCTCTGAACGGTATTTTTCGTGAGACCAAGTCTCT10.12453300124533001No Hit
GCTTAAAAACAAGGCAGCTCTGAACGGTATTTTTCGTGAGACCAAGTCTC10.12453300124533001No Hit
ACTTAAAAACAAGGCAGTGGTATCAACGCAGAGTACATGGGGAGCAGTGG10.12453300124533001Clontech SMARTer II A Oligonucleotide (96% over 25bp)
ACTTAAAAACAAGGCAGCTCTGAACGGTTCGTGAGACCAAGTCTCTGCTA10.12453300124533001No Hit
ACTTAAAACAAGGCAGTGCTATCAACGAAGAGGTCCATTCTAGTGCCTTC10.12453300124533001No Hit

[FAIL]Adapter Content

Adapter graph