FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151618

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151618
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10202
Total Bases1.4 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC241223.642423054303077No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC217921.358557145657713No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC177217.369143305234267No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC9859.654969613801216No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG2492.440697902372084No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA660.646931974122721No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA620.607723975691041No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT420.4116839835326407No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA400.3920799843168006No Hit
AAATACCGTTCAGAGCTGCCATGTCATGAGTGGCCAGATGGAGAGGCCCA380.3724759851009606No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA380.3724759851009606No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA360.35287198588512053No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA360.35287198588512053No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA350.34306998627720053No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG350.34306998627720053No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA340.33326798666928054No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT330.3234659870613605No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT320.3136639874534405No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA310.3038619878455205No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG300.2940599882376005No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA260.25485198980592044No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA230.2254459909821604No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT230.2254459909821604No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT220.21564399137424034No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA220.21564399137424034No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA210.20584199176632034No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA210.20584199176632034No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA180.17643599294256027No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGACCC150.14702999411880024No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA150.14702999411880024No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGACCC150.14702999411880024No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT150.14702999411880024No Hit
GGCACTAGAATGGACCTCTTCGTCATCACTAAATTATTTCAATTAAATAC140.13722799451088022No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA140.13722799451088022No Hit
AAATACCGTTCAGAGCTGCCCCCAAGAGCTTGCAAATTGCTGCTGGAACC140.13722799451088022No Hit
GGCACTAGAATGGCCTCTTCCTCATCACTAAATTATTTCAATTAAATACA140.13722799451088022No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA130.12742599490296022No Hit
GGCACTAGAATGGACCCTTCCTCATCACTAAATTATTTCAATTAAATACA120.1176239952950402No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTTT120.1176239952950402No Hit
AAATACCGTTCAAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT110.10782199568712017No Hit
AAATACCGTTCAAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT110.10782199568712017No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)