FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151620

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151620
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9473
Total Bases1.3 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC348536.7887680776945No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC227824.04729230444421No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC8679.152327668109365No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG5495.7954185580069675No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG1631.7206798268763854No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA850.8972870262852317No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG610.6439353953341075No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT510.538372215771139No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA490.5172595798585453No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA360.3800274464266864No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA360.3800274464266864No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA300.3166895386889053No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT290.30613322073260846No Hit
AAATACCGTTCAGAGCGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT270.2850205848200148No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTTT260.27446426686371794No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA210.22168267708223371No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA210.22168267708223371No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT190.20057004116964No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA190.20057004116964No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCTTCCATGTACTCTGC190.20057004116964Clontech SMARTer II A Oligonucleotide (95% over 22bp)
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGTAAATTGCTGCTGGAACC180.1900137232133432No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGACCC180.1900137232133432No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACATTACGCTGTAGAA170.17945740525704634No Hit
AAATCCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT160.1689010873007495No Hit
AAATACCGTTAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT160.1689010873007495No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA160.1689010873007495No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA140.1477884513881558No Hit
AAATACCGTTCGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT130.13723213343185897No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTCGCAAATTGCTGCTGGAACC120.12667581547556211No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTTTACGCTGTAGAACCATGT120.12667581547556211No Hit
GGCACTAGAATGGACCCTTCCTCATCACTAAATTATTTCAATTAAATACA120.12667581547556211No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT120.12667581547556211No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA110.11611949751926527No Hit
GGCACTAGAATGGACCTCTGCGTTGATACCACTGCTTCCATGTACTCTGC110.11611949751926527Clontech Universal Primer Mix Long (95% over 23bp)
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCCGCTGGAACC110.11611949751926527No Hit
AAAACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT100.10556317956296843No Hit
AAATACCGTTCAGGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT100.10556317956296843No Hit
AAATACCGTTCAAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT100.10556317956296843No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)