FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151624

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151624
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14081
Total Bases2 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC385527.377316951921028No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC307221.816632341453023No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC207014.70066046445565No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC13609.658404942830764No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG5223.7071230736453376No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG1581.1220793977700447No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA1250.8877210425395923No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA830.5894467722462893No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA820.5823450039059725No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA510.36219018535615366No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG480.3408848803352035No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA460.32668134365456997No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA460.32668134365456997No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA410.2911725019529863No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA400.28407073361266955No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA360.2556636602514026No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA350.24856189191108588No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA340.24146012357076913No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA330.2343583552304524No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT330.2343583552304524No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA330.2343583552304524No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA310.22015481854981891No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA270.19174774518855195No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA270.19174774518855195No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA270.19174774518855195No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA250.17754420850791847No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT250.17754420850791847No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT230.16334067182728498No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA190.13493359846601805No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA180.1278318301257013No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA170.12073006178538456No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA160.11362829344506782No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTTT160.11362829344506782No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT150.10652652510475108No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)