FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151642

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151642
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10818
Total Bases1.5 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC534849.43612497689037No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC155514.374191162876688No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC7647.062303568127196No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG3853.5588833425771864No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA1571.451284895544463No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA1531.4143094841930115No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG1221.1277500462192642No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC880.8134590497319282No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA760.7025328156775744No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA710.6563135514882603No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA630.5823627287853578No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA570.5268996117581808No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA530.4899242004067295No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA470.43446108337955264No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG400.36975411351451287No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA380.3512664078387872No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA280.258827879460159No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTTT280.258827879460159No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT270.24958402662229617No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT240.22185246810870773No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA220.20336476243298204No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTACATTATTTCAATTAAATAC200.18487705675725644No Hit
GGCACTAGAATGGACCCTTCCTCATCACTACATTATTTCAATTAAATACA190.1756332039193936No Hit
GGCACTAGAATGGACCTCTTCGTCATCACTACATTATTTCAATTAAATAC170.15714549824366797No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT170.15714549824366797No Hit
GGCACTAGAATGGACCCCTTCCTCATCACTACATTATTTCAATTAAATAC160.14790164540580514No Hit
GGCACTAGAATGGACTTCTTCCTCATCACTACATTATTTCAATTAAATAC160.14790164540580514No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACATTACGCTGTAGAA150.13865779256794233No Hit
GGCACTAGAATGGACCTCTCCCTCATCACTACATTATTTCAATTAAATAC150.13865779256794233No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCTTCCCATGTACTCTG150.13865779256794233Clontech SMARTer II A Oligonucleotide (95% over 22bp)
GGCACTAGAATGGACCTCGTCCTCATCACTACATTATTTCAATTAAATAC140.1294139397300795No Hit
GGCACTAGAATGGACCTCTTACTCATCACTACATTATTTCAATTAAATAC130.12017008689221668No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTAAATTAAATAC130.12017008689221668No Hit
GGCACTAGAATGGACCTCTTCCCCATCACTACATTATTTCAATTAAATAC120.11092623405435387No Hit
GGCACTAGAATGGACCTCTTCCTCACCACTACATTATTTCAATTAAATAC120.11092623405435387No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCTTCCATGTACTCTGC110.10168238121649102Clontech SMARTer II A Oligonucleotide (95% over 22bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)