FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151654

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151654
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13563
Total Bases1.9 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC356626.292118262921182No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC321523.704195237041954No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC247118.218683182186833No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC8015.90577305905773No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG3142.31512202315122No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA1010.74467300744673No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA980.7225540072255401No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA840.6193320061933201No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT750.5529750055297501No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT670.49399100493991005No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG620.45712600457126No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA510.37602300376023007No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA490.36127700361277004No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA470.34653100346531No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA440.32441200324412006No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA390.28754700287547No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA380.28017400280174No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA370.27280100272801006No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA370.27280100272801006No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA350.25805500258055003No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA340.25068200250682005No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA340.25068200250682005No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA330.24330900243309003No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA330.24330900243309003No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTTT320.23593600235936002No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA300.22119000221190005No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG300.22119000221190005No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA280.20644400206444002No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT280.20644400206444002No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA240.17695200176952003No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA230.16957900169579002No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT220.16220600162206003No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGACCC170.12534100125341002No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA160.11796800117968001No Hit
AAATACCGTTCAAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT150.11059500110595002No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA140.10322200103222001No Hit
GGCACTAGAATGGACCTCTTCGTCATCACTACATTATTTCAATTAAATAC140.10322200103222001No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)