FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151662

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151662
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12890
Total Bases1.8 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC345526.803723816912335No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC274221.272304111714508No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC227017.61055081458495No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC9727.540729247478666No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG4053.1419705197827774No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT1291.0007757951900698No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG910.7059736229635376No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA860.6671838634600465No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA790.612878200155159No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT780.6051202482544608No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA680.5275407292474786No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA460.3568657874321179No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA410.3180760279286268No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG400.3103180760279286No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA350.27152831652443754No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA340.2637703646237393No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA330.2560124127230411No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA330.2560124127230411No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA320.24825446082234287No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT280.21722265321955006No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA280.21722265321955006No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA270.20946470131885184No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA230.17843289371605894No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA220.17067494181536075No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA220.17067494181536075No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA220.17067494181536075No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA200.1551590380139643No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT180.13964313421256788No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA180.13964313421256788No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA170.13188518231186966No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT160.12412723041117144No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA160.12412723041117144No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA160.12412723041117144No Hit
GGCACTAGAATGGACCTCTTCCTCACCACTAAATTATTTCAATTAAATAC150.11636927851047324No Hit
AAATACCGTTCAGAGCTGCCCCCAGGAGCTTGCAAATTGCTGCTGGAACC150.11636927851047324No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT130.10085337470907679No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)