FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151682

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151682
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12752
Total Bases1.8 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC435934.18287327478043No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC216817.00125470514429No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC206716.20922208281054No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC8226.446047678795483No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG3142.4623588456712673No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA1301.019447929736512No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG790.6195106649937265No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA790.6195106649937265No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA670.5254077791718946No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA610.47835633626097873No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA520.4077791718946048No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTACTGCTGGAACC520.4077791718946048No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA470.3685696361355082No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA470.3685696361355082No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA440.3450439146800502No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA410.3215181932245922No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA360.2823086574654956No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA350.2744667503136763No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA350.2744667503136763No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG340.266624843161857No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTTT320.2509410288582183No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT310.243099121706399No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT290.22741530740276036No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA280.21957340025094102No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT250.19604767879548307No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA230.1803638644918444No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA230.1803638644918444No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA220.1725219573400251No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA220.1725219573400251No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA200.15683814303638646No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT180.1411543287327478No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA180.1411543287327478No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTACATTATTTCAATTAAATAC140.10978670012547051No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA140.10978670012547051No Hit
GGCACTAGAATGGACCTCTCCCTCATCACTACATTATTTCAATTAAATAC140.10978670012547051No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACACTATTTCAATTAAATAC130.1019447929736512No Hit
GGCACTAGAATGGACCTCTTCCCCATCACTACATTATTTCAATTAAATAC130.1019447929736512No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGACCC130.1019447929736512No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)