FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151686

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151686
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14841
Total Bases2.1 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC411927.75419446128967No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC361724.37167306785257No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC206813.93437099925881No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC12688.543898659120005No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG4553.065831143453945No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA1270.8557374839970353No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG1050.7074994946432182No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA970.6535947712418301No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA790.532309143588707No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA760.5120948723131864No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA570.3840711542348898No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA560.3773330638097163No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA530.3571187925341958No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA420.28299979785728724No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA400.2695236170069402No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA400.2695236170069402No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA390.26278552658176674No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA380.2560474361565932No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA380.2560474361565932No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA350.2358331648810727No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA340.22909507445589922No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG340.22909507445589922No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA330.22235698403072568No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT320.2156188936055522No Hit
AAATACCGTTCAGAGCTGCCATGTCATGAGTGGCCAGATGGAGAGGCCCA260.17519035105451117No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA260.17519035105451117No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA260.17519035105451117No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA250.16845226062933766No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA250.16845226062933766No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT220.14823798935381713No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA180.1212856276531231No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA170.11454753722794961No Hit
GGCACTAGAATGGACCCTTCCTCATCACTAAATTATTTCAATTAAATACA150.1010713563776026No Hit
GGCACTAGAATGGACCTCTCCCTCATCACTAAATTATTTCAATTAAATAC150.1010713563776026No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)