FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151696

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151696
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12537
Total Bases1.8 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC355328.340113264736384No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC261820.8821887213847No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC222817.771396665869027No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC6815.431921512323522No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG4053.230437903804738No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG1150.9172848368828269No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA1010.8056153784797002No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT720.574300071787509No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA620.4945361729281327No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA580.46263061338438227No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG560.446677833612507No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA480.382866714525006No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA420.33500837520938026No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA410.32703198532344263No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA410.32703198532344263No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT390.3110792055515674No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT360.2871500358937545No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA330.2632208662359416No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA310.24726808646406634No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA310.24726808646406634No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA300.23929169657812877No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA280.2233389168062535No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA260.20738613703437822No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA250.1994097471484406No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA240.191433357262503No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA240.191433357262503No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA240.191433357262503No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA230.18345696737656536No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA210.16750418760469013No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA210.16750418760469013No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT190.15155140783281484No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT180.14357501794687724No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTTT180.14357501794687724No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACATTACGCTGTAGAA160.12762223817500198No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA160.12762223817500198No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTACATTATTTCAATTAAATAC140.11166945840312675No Hit
GGCACTAGAATGGACCCTTCCTCATCACTACATTATTTCAATTAAATACA130.10369306851718911No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)