FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151702

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151702
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14580
Total Bases2.1 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC439230.12345679012346No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC321822.071330589849108No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC234916.11111111111111No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC7495.137174211248285No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG4583.1412894375857343No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG1170.8024691358024691No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA1000.6858710562414266No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA990.6790123456790124No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA890.6104252400548696No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT830.569272976680384No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA660.45267489711934156No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA520.35665294924554186No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGAAAGCCAATGGGAAA520.35665294924554186No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA520.35665294924554186No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA460.31550068587105623No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA440.30178326474622774No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA420.2880658436213992No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT420.2880658436213992No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG420.2880658436213992No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA380.2606310013717421No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA370.25377229080932784No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA330.22633744855967078No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA320.21947873799725653No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA300.205761316872428No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA290.19890260631001372No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA280.19204389574759945No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA270.1851851851851852No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA270.1851851851851852No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTTT260.17832647462277093No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA250.17146776406035666No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT230.15775034293552812No Hit
AAATACCGTTCAAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT170.11659807956104253No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA160.10973936899862827No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACATTACGCTGTAGAA160.10973936899862827No Hit
AAATACCGTTAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT160.10973936899862827No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT150.102880658436214No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)