FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151708

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151708
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11720
Total Bases1.6 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC608351.90273037542662No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC209217.84982935153584No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG3312.8242320819112625No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA1841.5699658703071673No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC1621.3822525597269624No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA1471.2542662116040957No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT1241.0580204778156996No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG940.8020477815699658No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT930.7935153583617748No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA910.7764505119453925No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA740.6313993174061433No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA730.6228668941979522No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA710.6058020477815699No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA700.5972696245733788No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA660.5631399317406144No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG590.5034129692832764No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA330.2815699658703072No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA320.27303754266211605No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA290.24744027303754265No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTTT280.2389078498293515No Hit
GGCACTAGAATGGACCTCTTCGTCATCACTACATTATTTCAATTAAATAC270.23037542662116042No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT250.21331058020477817No Hit
GGCACTAGAATGGACCTCTCCCTCATCACTACATTATTTCAATTAAATAC230.1962457337883959No Hit
GGCACTAGAATGGCCTCTTCCTCATCACTACATTATTTCAATTAAATACA230.1962457337883959No Hit
GGCACTAGAATGGACCCTTCCTCATCACTACATTATTTCAATTAAATACA220.18771331058020477No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTACATTATTTCAATTAAATAC210.17918088737201365No Hit
GGCACTAGAATGGACCTCTTCCCCATCACTACATTATTTCAATTAAATAC170.14505119453924914No Hit
AAATACCGTTCAAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT150.12798634812286688No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGACCC150.12798634812286688No Hit
GGCACTAGAATGGACCTCTTCCTCACCACTACATTATTTCAATTAAATAC150.12798634812286688No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATGC130.11092150170648465No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACATTACGCTGTAGAA130.11092150170648465No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT130.11092150170648465No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGAAACC130.11092150170648465No Hit
GGCACTAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACAA120.10238907849829351No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATCTCAATTAAATAC120.10238907849829351No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)