FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151710

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151710
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9259
Total Bases1.3 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC286130.899665190625335No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC209122.58343233610541No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC172618.641321957014796No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG3143.391294956258775No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG810.8748244950858624No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA800.8640241926773949No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA650.7020196565503835No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT560.6048169348741764No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT470.5076142131979695No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA420.45361270115563235No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA400.43201209633869747No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA330.35640997947942543No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG330.35640997947942543No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT320.345609677070958No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA270.2916081650286208No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA250.2700075602116859No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA250.2700075602116859No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT200.21600604816934874No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTTT200.21600604816934874No Hit
AAATACCGTTCAAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT180.19440544335241386No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA170.18360514094394642No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA160.172804838535479No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTACATTATTTCAATTAAATAC160.172804838535479No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT160.172804838535479No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT150.16200453612701154No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC150.16200453612701154No Hit
GGCACTAGAATGGACCCTTCCTCATCACTACATTATTTCAATTAAATACA130.14040393131007667No Hit
AAATCCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT120.12960362890160926No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA120.12960362890160926No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT110.11880332649314182No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATGC100.10800302408467437No Hit
AAATACCGTTAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT100.10800302408467437No Hit
GGCACTAGAATGGACCTCTCCCTCATCACTACATTATTTCAATTAAATAC100.10800302408467437No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)