FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151724

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151724
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10902
Total Bases1.5 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC282325.89433131535498No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC197218.088424142359198No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC149713.731425426527244No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC9178.411300678774538No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG5344.898183819482664No Hit
AAATACCGTTCAGAGCTGCCACCAAGAACTTGCAAATTGCTGCTGGAACC4964.549623922216107No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG1591.4584479911942763No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA830.761328196661163No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA660.6053935057787562No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG600.5503577325261421No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA420.3852504127682994No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT410.37607778389286367No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA370.3393872683911209No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA360.3302146395156852No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA330.3026967528893781No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT320.2935241240139424No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA290.2660062373876353No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA290.2660062373876353No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA250.2293157218858925No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA250.2293157218858925No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA230.21097046413502107No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA210.1926252063841497No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA200.183452577508714No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA200.183452577508714No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA190.1742799486332783No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT180.1651073197578426No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA170.1559346908824069No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGACCC170.1559346908824069No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT150.13758943313153552No Hit
AAATACCGTTCAAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT140.1284168042560998No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA140.1284168042560998No Hit
GGCACTAGAATGGACCTCTTCCCCATCACTAAATTATTTCAATTAAATAC140.1284168042560998No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTTT140.1284168042560998No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA130.1192441753806641No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA130.1192441753806641No Hit
AAATACCGTTCAGAGCTGCCCCCAAGAGCTTGCAAATTGCTGCTGGAACC110.10089891762979271No Hit
GGCACTAGAATGGACCCTTCCTCATCACTAAATTATTTCAATTAAATACA110.10089891762979271No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)