FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151728

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151728
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10714
Total Bases1.5 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC288126.890050401344034No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC247223.072615269740528No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC210119.609856262833674No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG4824.498786634310249No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG1631.521373903304088No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA970.9053574761993654No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA750.7000186671644577No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA640.5973492626470039No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA450.4200112002986746No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT420.39201045361209635No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA410.3826768713832369No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA380.3546761246966586No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG380.3546761246966586No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA370.34534254246779916No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA360.3360089602389397No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA300.2800074668657831No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT280.2613403024080642No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA280.2613403024080642No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA270.2520067201792048No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA270.2520067201792048No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACATTACGCTGTAGAA260.24267313795034534No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA250.2333395557214859No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGAAAGCCAATGGGAAA240.22400597349262646No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT230.21467239126376705No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA230.21467239126376705No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT220.20533880903490762No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA210.19600522680604818No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA210.19600522680604818No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA200.18667164457718874No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA190.1773380623483293No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTTT170.15867089789061042No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA150.14000373343289155No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA150.14000373343289155No Hit
GGCACTAGAATGGACCTCTCCCTCATCACTAAATTATTTCAATTAAATAC140.1306701512040321No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGACCC130.12133656897517267No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTAAATTATTTCAATTAAATAC130.12133656897517267No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTACATTATTTCAATTAAATAC120.11200298674631323No Hit
GGCACTAGAATGGACCTCTTCCTCACCACTAAATTATTTCAATTAAATAC120.11200298674631323No Hit
AAATACCGTTCAGAGCTGCCCCCAGGAGCTTGCAAATTGCTGCTGGAACC120.11200298674631323No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGATGCTGGAACC110.10266940451745381No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA110.10266940451745381No Hit
AAATACCGTTCAGAGCGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT110.10266940451745381No Hit
AAATACCGTTCAGAGCTGCCACCCACAGAAACACACACACATTACGCTGT110.10266940451745381No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT110.10266940451745381No Hit
AAATACCGTTCAAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT110.10266940451745381No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)