FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151742

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151742
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12232
Total Bases1.7 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC488739.95258338783519No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC255920.92053629823414No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC10238.363309352517986No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC4954.046762589928058No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG3552.9022236756049704No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA1331.0873119686069326No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG980.8011772400261609No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA930.7603008502289078No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA650.5313930673642904No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA600.49051667756703726No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT550.44964028776978415No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA530.43328973185088293No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA520.4251144538914323No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA520.4251144538914323No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT510.4169391759319817No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA490.40058862001308043No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA460.3760627861347286No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG460.3760627861347286No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT340.27795945062132116No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA300.24525833878351863No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA300.24525833878351863No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTACATTATTTCAATTAAATAC220.1798561151079137No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGACCC200.1635055591890124No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTTT190.1553302812295618No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT170.13897972531066058No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT170.13897972531066058No Hit
GGCACTAGAATGGCCTCTTCCTCATCACTACATTATTTCAATTAAATACA170.13897972531066058No Hit
GGCACTAGAATGGACCTCTTCCCCATCACTACATTATTTCAATTAAATAC150.12262916939175932No Hit
AAATACCGTTCAAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT150.12262916939175932No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA140.11445389143230869No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA140.11445389143230869No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)