FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151744

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151744
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11963
Total Bases1.7 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC504942.205132491849874No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC239620.028420964640976No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC131611.000585137507315No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG3282.7417871771294826No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA1441.2037114436178216No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA1150.9612973334447881No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG840.7021650087770626No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT710.5934966145615648No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA670.5600601855721808No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA590.49318732759341305No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA560.4681100058513751No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA560.4681100058513751No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA460.38451893337791526No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT400.3343642898938393No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA390.32600518264649336No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT390.32600518264649336No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA350.29256875365710944No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA290.24241411017303355No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT280.23405500292568754No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG270.22569589567834153No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA250.2089776811836496No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTTT230.19225946668895763No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT210.17554125219426564No Hit
GGCACTAGAATGGACCCTTCCTCATCACTACATTATTTCAATTAAATACA180.1504639304522277No Hit
GGCACTAGAATGGACCTCTTCGTCATCACTACATTATTTCAATTAAATAC170.14210482320488174No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTACATTATTTCAATTAAATAC170.14210482320488174No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT160.13374571595753573No Hit
AAATACCGTTAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT140.11702750146284377No Hit
AAATACCGTTCAGAGCGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT140.11702750146284377No Hit
GGCACTAGAATGGCCTCTTCCTCATCACTACATTATTTCAATTAAATACA140.11702750146284377No Hit
GGCACTAGAATGGACCTCTTCCTCACCACTACATTATTTCAATTAAATAC120.10030928696815179No Hit
AAATACCGTTCAAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT120.10030928696815179No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)