FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151750

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151750
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13279
Total Bases1.9 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC339425.55915355071918No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC289121.771217712177123No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC265319.978914074855034No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC9687.289705550116726No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG2802.1085925144965736No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT2101.58144438587243No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT1431.0768883199036072No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA890.6702311921078394No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA770.5798629414865577No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA710.5346788161759168No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG550.4141878153475412No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA520.39159575269222086No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT400.30122750207093907No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA390.29369681451916557No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA380.28616612696739213No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA350.2635740643120717No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA300.2259206265532043No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA290.2183899390014308No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG290.2183899390014308No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTTT270.20332856389788387No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA260.19579787634611043No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA250.18826718879433693No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA250.18826718879433693No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA240.18073650124256346No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA230.17320581369078997No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA230.17320581369078997No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA220.1656751261390165No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT220.1656751261390165No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA210.158144438587243No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA200.15061375103546953No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT200.15061375103546953No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA200.15061375103546953No Hit
AAATACCGTTCAAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT190.14308306348369607No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA190.14308306348369607No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGACCC170.1280216883801491No Hit
GGCACTAGAATGGACCTCTTCGTCATCACTAAATTATTTCAATTAAATAC140.10542962572482868No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA140.10542962572482868No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)