FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151774

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151774
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8273
Total Bases1.1 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC315938.18445545751239No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC125015.109391998065998No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC111713.501752689471774No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG3203.8680043515048954No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC1271.5351142270035054No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA1061.2812764414359965No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG961.1604013054514686No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT901.0878762238607518No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA831.0032636286715821No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG620.7494258431040735No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT560.6769007615133567No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA510.6164631935210927No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA500.60437567992264No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA490.5922881663241871No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA470.5681131391272816No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA410.49558805753656476No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA400.4835005439381119No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA350.4230629759458479No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA310.3747129215520367No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA230.27801281276441436No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA230.27801281276441436No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA210.2538377855675088No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCTTCCATGTACTCTGC210.2538377855675088Clontech SMARTer II A Oligonucleotide (95% over 22bp)
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA190.22966275837060318No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA180.21757524477215034No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA170.20548773117369756No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA170.20548773117369756No Hit
GGCACTAGAATGGACCCTTCCTCATCACTACATTATTTCAATTAAATACA130.15713767677988638No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA120.14505016318143357No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT120.14505016318143357No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACATTACGCTGTAGAA110.13296264958298076No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT110.13296264958298076No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA110.13296264958298076No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA100.12087513598452798No Hit
GGCACTAGAATGGACCCCTTCCTCATCACTACATTATTTCAATTAAATAC100.12087513598452798No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA100.12087513598452798No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA100.12087513598452798No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTTT100.12087513598452798No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTACATTATTTCAATTAAATAC90.10878762238607517No Hit
GGCACTAGAATGGCCTCTTCCTCATCACTACATTATTTCAATTAAATACA90.10878762238607517No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)