FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151780

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151780
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5175
Total Bases750.3 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC357369.04347826086956No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG2735.27536231884058No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG531.0241545893719806No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG470.9082125603864735No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT470.9082125603864735No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT260.5024154589371981No Hit
AAATACCGTTCAGAGCTGCCCCCAAGAGCTTGCAAATTGCTGCTGGAACC250.4830917874396135No Hit
AAATACCGTTCAAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT230.4444444444444444No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGACCC230.4444444444444444No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT220.4251207729468599No Hit
AAATACCGTTCGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT180.34782608695652173No Hit
AAATACCGTTCAGAGCGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT180.34782608695652173No Hit
AAATACCGTTCAGAGCTGCCACCAAGGGCTTGCAAATTGCTGCTGGAACC160.30917874396135264No Hit
AAATACCTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT150.2898550724637681No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGATGCTGGAACC150.2898550724637681No Hit
AAATACCGTTCAGAGCTGCCATGTCATGAGTGGCCAGATAGAGAGGCCCA140.2705314009661836No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGATTGCAAATTGCTGCTGGAACC140.2705314009661836No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTTT130.25120772946859904No Hit
AAATACCGTTCAGGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT130.25120772946859904No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGTTTGCAAATTGCTGCTGGAACC120.2318840579710145No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCCGGAACC110.21256038647342995No Hit
AAATACCGTTCAGAGCTGCCGCCAAGAGCTTGCAAATTGCTGCTGGAACC100.1932367149758454No Hit
AAATACCGTTAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT100.1932367149758454No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCGAATTGCTGCTGGAACC100.1932367149758454No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTTCAAATTGCTGCTGGAACC90.17391304347826086No Hit
AAATACCGTTCAGAGTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT90.17391304347826086No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATAGCTGCTGGAACC90.17391304347826086No Hit
AAATCCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT80.15458937198067632No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAGCC80.15458937198067632No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT80.15458937198067632No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTTCTGCTGGAACC70.1352657004830918No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCCGCTGGAACC70.1352657004830918No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTTTACGCTGTAGAACCATGT70.1352657004830918No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACATTACGCTGTAGAA70.1352657004830918No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGCACC70.1352657004830918No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGTTGGAACC70.1352657004830918No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCAGCTGGAACC70.1352657004830918No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC70.1352657004830918No Hit
AAATACCGTTAAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC70.1352657004830918No Hit
AAATACAGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC70.1352657004830918No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT70.1352657004830918No Hit
GGCACTAGAATGGACCTCTTCCGCCCAGCCCCAGTTACCAGGCAGTCCCC60.11594202898550725No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTTGAACC60.11594202898550725No Hit
AAATACCGTTCAGAGCAGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC60.11594202898550725No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATCGCTGCTGGAACC60.11594202898550725No Hit
AAATACCGTTCAGAGCTGCCACCGAGAGCTTGCAAATTGCTGCTGGAACC60.11594202898550725No Hit
AAATACCGTTCAGAGCTGCCACAAAGAGCTTGCAAATTGCTGCTGGAACC60.11594202898550725No Hit
AAATACCGTTCAGAGCTGCCACCAAGAACTTGCAAATTGCTGCTGGAACC60.11594202898550725No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCTTCCATGTACTCTGC60.11594202898550725Clontech SMARTer II A Oligonucleotide (95% over 22bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)