FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151784

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151784
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10312
Total Bases1.4 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC291828.29712955779674No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC254724.699379363847946No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC132312.829712955779673No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC6035.84755624515128No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG4033.9080682699767264No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG1181.144297905352987No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA950.9212567882079131No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA730.7079131109387121No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG570.5527540729247479No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA500.48487199379363843No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA420.4072924747866563No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT390.37820015515903804No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA360.3491078355314197No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA350.3394103956555469No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA330.3200155159038014No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA310.30062063615205586No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT280.27152831652443754No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA230.22304111714507371No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA220.21334367726920092No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA220.21334367726920092No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGACCC220.21334367726920092No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT220.21334367726920092No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA190.18425135764158262No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT160.1551590380139643No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA140.13576415826221877No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTTT140.13576415826221877No Hit
AAATACCGTTCAGAGCGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT140.13576415826221877No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGATGCTGGAACC120.11636927851047324No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT120.11636927851047324No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACATTACGCTGTAGAA120.11636927851047324No Hit
AAATACCGTTAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT120.11636927851047324No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA110.10667183863460046No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA110.10667183863460046No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)