FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151786

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151786
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9240
Total Bases1.3 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC653870.75757575757575No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG2552.75974025974026No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA1892.0454545454545454No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA1451.5692640692640691No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG1001.0822510822510822No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA971.0497835497835497No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA830.8982683982683983No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA790.8549783549783551No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA750.8116883116883116No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA710.7683982683982684No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA530.5735930735930735No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA530.5735930735930735No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG520.5627705627705628No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA440.4761904761904762No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA410.44372294372294374No Hit
GGCACTAGAATGGACCCTTCCTCATCACTAAATTATTTCAATTAAATACA310.3354978354978355No Hit
GGCACTAGAATGGCCTCTTCCTCATCACTAAATTATTTCAATTAAATACA260.2813852813852814No Hit
GGCACTAGAATGGACCCCTTCCTCATCACTAAATTATTTCAATTAAATAC240.2597402597402597No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCTTCCATGTACTCTGC220.2380952380952381Clontech SMARTer II A Oligonucleotide (95% over 22bp)
AAATACCGTTCAGAGCTGCCACAGAAACACACACACATTACGCTGTAGAA200.21645021645021645No Hit
GGCACTAGAATGGACCTCTTCCCCATCACTAAATTATTTCAATTAAATAC200.21645021645021645No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTAAATTATTTCAATTAAATAC200.21645021645021645No Hit
GGCACTAGAATGGACCTCTTCGTCATCACTAAATTATTTCAATTAAATAC180.19480519480519481No Hit
GGCACTAGAATGGACCTCTCCCTCATCACTAAATTATTTCAATTAAATAC180.19480519480519481No Hit
GGCACTAGAATGGACCTCCTCCTCATCACTAAATTATTTCAATTAAATAC160.17316017316017315No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTTT160.17316017316017315No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCTCCCATGTACTCTGC160.17316017316017315Clontech SMARTer II A Oligonucleotide (95% over 21bp)
GGCACTAGAATGGACCTCTGCGTTGATACCACTGCTTCCATGTACTCTGC150.16233766233766234Clontech Universal Primer Mix Long (95% over 23bp)
GGCACTAGAATGGACTTCTTCCTCATCACTAAATTATTTCAATTAAATAC150.16233766233766234No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAAATAAATAC130.1406926406926407No Hit
GGCACTAGAATGGACCTCTTCCTCACCACTAAATTATTTCAATTAAATAC130.1406926406926407No Hit
GGCACTAGAATGGACCTCTTACTCATCACTAAATTATTTCAATTAAATAC120.12987012987012986No Hit
GGCACTAGAATGGACCTTTTCCTCATCACTAAATTATTTCAATTAAATAC110.11904761904761905No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCGATTAAATAC100.10822510822510822No Hit
GGCACTAGAATGGATCTCTTCCTCATCACTAAATTATTTCAATTAAATAC100.10822510822510822No Hit
GGCACTAGAATGGACCTCTTCCTCATCGCTAAATTATTTCAATTAAATAC100.10822510822510822No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)