FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151792

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151792
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12649
Total Bases1.8 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC379129.970748675784648No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC299923.709384141038818No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC215217.013202624713415No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG4323.4152897462249983No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC2011.5890584235907976No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG1160.9170685429678236No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA1050.8301051466519093No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA870.6878014072258677No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA580.4585342714839118No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG480.37947663846944424No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA470.37157087516799747No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA430.33994782196221046No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT390.3083247687564234No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA380.3004190054549767No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA360.28460747885208315No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA350.2767017155506364No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA340.2687959522491897No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA310.24507866234484943No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA300.23717289904340266No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA290.2292671357419559No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA270.2134556091390624No Hit
GGCACTAGAATGGACCTCTTCGTCATCACTAAATTATTTCAATTAAATAC260.20554984583761562No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT250.19764408253616886No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA250.19764408253616886No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA240.18973831923472212No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA230.18183255593327535No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA220.17392679263182861No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT220.17392679263182861No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA210.16602102933038185No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGACCC210.16602102933038185No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA210.16602102933038185No Hit
AAATACCGTTCAAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT200.15811526602893508No Hit
GGCACTAGAATGGACCCCTTCCTCATCACTAAATTATTTCAATTAAATAC190.15020950272748834No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA190.15020950272748834No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTTT170.13439797612459484No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT160.12649221282314807No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGGACC150.11858644952170133No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA150.11858644952170133No Hit
AAATACCTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT150.11858644952170133No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT140.11068068622025456No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)