FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151812

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151812
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13123
Total Bases1.9 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC369228.13381086641774No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC243318.539967995123067No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC204815.6061876095405No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC140910.736874190352816No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG3132.3851253524346565No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA1130.861083593690467No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT1060.8077421321344205No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT890.6781985826411644No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG860.6553379562600015No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA710.5410348243541873No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA540.4114912748609312No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA540.4114912748609312No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA540.4114912748609312No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA420.32004876933627985No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG390.297188142955117No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA380.28956793416139603No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA370.28194772536767504No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA370.28194772536767504No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA340.2590870989865123No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT290.22098605501790747No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTTT280.21336584622418656No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA270.2057456374304656No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA270.2057456374304656No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA270.2057456374304656No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA270.2057456374304656No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA250.1905052198430237No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA240.18288501104930274No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT240.18288501104930274No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA230.1752648022555818No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA220.16764459346186086No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA200.15240417587441896No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA190.14478396708069802No Hit
GGCACTAGAATGGACCTCTTCGTCATCACTACATTATTTCAATTAAATAC180.13716375828697708No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGACCC180.13716375828697708No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT150.11430313190581422No Hit
GGCACTAGAATGGACCCTTCCTCATCACTACATTATTTCAATTAAATACA150.11430313190581422No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA140.10668292311209328No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)