FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151814

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151814
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8281
Total Bases1.2 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC239528.921627822726727No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC165019.925129815239707No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC150918.222436903755586No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG3834.625045284385944No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC1952.3547880690737837No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG911.098901098901099No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA740.8936118826228715No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA590.7124743388479652No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG530.6400193213380027No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA410.4951092863180775No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT400.4830334500664171No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA350.422654268808115No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA320.38642676005313364No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA320.38642676005313364No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTTT290.3501992512981524No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT290.3501992512981524No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA260.3139717425431711No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA250.3018959062915107No Hit
GGCACTAGAATGGACCTCTTCGTCATCACTACATTATTTCAATTAAATAC220.26566839753652943No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA210.25359256128486896No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT210.25359256128486896No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT180.2173650525298877No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT170.20528921627822727No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT160.19321338002656682No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGACCC140.16906170752324598No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA130.15698587127158556No Hit
GGCACTAGAATGGACCTCTCCCTCATCACTACATTATTTCAATTAAATAC130.15698587127158556No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA120.14491003501992514No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTACATTATTTCAATTAAATAC110.13283419876826472No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT110.13283419876826472No Hit
AAATACCGTTCAAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT110.13283419876826472No Hit
AAATACCGTTAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT100.12075836251660428No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATGC100.12075836251660428No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGACCC90.10868252626494385No Hit
GGCACTAGAATGGACCCCTTCCTCATCACTACATTATTTCAATTAAATAC90.10868252626494385No Hit
AAATACCGTTCAGAGCTGCCCCCAGGAGCTTGCAAATTGCTGCTGGAACC90.10868252626494385No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATGT90.10868252626494385No Hit
GGCACTAGAATGGACTTCTTCCTCATCACTACATTATTTCAATTAAATAC90.10868252626494385No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)