FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151816

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151816
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11145
Total Bases1.6 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC483643.391655450874836No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC224120.107671601615074No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC9638.640646029609691No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG3312.9699416778824586No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA1511.3548676536563482No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA970.870345446388515No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG860.7716464782413638No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT660.5921938088829072No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA620.5563032750112158No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA620.5563032750112158No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA560.5024674742036788No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA490.439659039928219No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA460.4127411395244504No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT420.3768506056527591No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA410.36787797218483625No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG380.34096007178106774No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA320.2871242709735307No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA320.2871242709735307No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT310.2781516375056079No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC300.2691790040376851No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT250.22431583669807087No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTTT220.19739793629430236No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA210.18842530282637954No Hit
GGCACTAGAATGGACCTCTTCGTCATCACTACATTATTTCAATTAAATAC190.17048003589053387No Hit
GGCACTAGAATGGACCTCTTCCTCATCACCACATTATTTCAATTAAATAC180.16150740242261105No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTACATTATTTCAATTAAATAC170.1525347689546882No Hit
GGCACTAGAATGGACCCCTTCCTCATCACTACATTATTTCAATTAAATAC170.1525347689546882No Hit
GGCACTAGAATGGCCTCTTCCTCATCACTACATTATTTCAATTAAATACA170.1525347689546882No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACACTATTTCAATTAAATAC160.14356213548676536No Hit
GGCACTAGAATGGACCCTTCCTCATCACTACATTATTTCAATTAAATACA150.13458950201884254No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT150.13458950201884254No Hit
GGCACTAGAATGGACCTCTCCCTCATCACTACATTATTTCAATTAAATAC150.13458950201884254No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGACCC140.1256168685509197No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACATTACGCTGTAGAA140.1256168685509197No Hit
GGCACTAGAATGGACCTCTTCCTCATCGCTACATTATTTCAATTAAATAC120.10767160161507401No Hit
GGCACTAGAATGGACCTCTTCCCCATCACTACATTATTTCAATTAAATAC120.10767160161507401No Hit
GGCACTAGAATGGACCTCTTCTCATCACTACATTATTTCAATTAAATACA120.10767160161507401No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)