FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151822

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151822
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8239
Total Bases1.1 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC331840.27187765505523No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC200424.32334021119068No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG4165.04915645102561No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC3754.551523243112028No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG1271.5414492050006068No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT750.9103046486224056No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA720.8738924626775094No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG510.6190071610632358No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA440.5340453938584779No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT430.5219079985435126No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA290.35198446413399687No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT290.35198446413399687No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA270.32770967350406605No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGACCC270.32770967350406605No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA260.3155722781891006No Hit
AAATACCGTTCAGAGCTGCCCCCAAGAGCTTGCAAATTGCTGCTGGAACC260.3155722781891006No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA260.3155722781891006No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA250.3034348828741352No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT220.26702269692923897No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT210.2548853016142736No Hit
AAATACCGTTCAGAGCGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT190.23061051098434274No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA180.21847311566937735No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA180.21847311566937735No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA150.18206092972448112No Hit
AAATACCGTTCGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT150.18206092972448112No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCTTCCATGTACTCTGC140.16992353440951571Clontech SMARTer II A Oligonucleotide (95% over 22bp)
AAATACCGTTCAAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT120.1456487437795849No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACATTACGCTGTAGAA120.1456487437795849No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCCGCTGGAACC110.13351134846461948No Hit
AAATACCGTTAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT110.13351134846461948No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGGACC100.12137395314965409No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGCACC90.10923655783468868No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCCGGAACC90.10923655783468868No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA90.10923655783468868No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTACTGCTGGAACC90.10923655783468868No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTAGAACC90.10923655783468868No Hit
AAATACCGTTCAGAGTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT90.10923655783468868No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)