FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151848

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151848
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11040
Total Bases1.5 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC302527.400362318840582No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC243522.056159420289855No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC173915.7518115942029No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC8908.06159420289855No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG3403.0797101449275366No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG860.7789855072463768No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA780.7065217391304348No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA540.48913043478260876No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA540.48913043478260876No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA410.3713768115942029No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA380.3442028985507246No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA370.3351449275362319No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA360.32608695652173914No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA350.3170289855072464No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT340.30797101449275366No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG320.2898550724637681No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA290.26268115942028986No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA290.26268115942028986No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA280.25362318840579706No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA270.24456521739130438No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA260.2355072463768116No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT250.22644927536231885No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA240.21739130434782608No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA230.20833333333333334No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA220.19927536231884058No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT220.19927536231884058No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTTT220.19927536231884058No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA190.1721014492753623No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA180.16304347826086957No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT150.1358695652173913No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA150.1358695652173913No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA150.1358695652173913No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGACCC140.12681159420289853No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA130.1177536231884058No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA130.1177536231884058No Hit
GGCACTAGAATGGACCCCTTCCTCATCACTACATTATTTCAATTAAATAC130.1177536231884058No Hit
GGCACTAGAATGGACCTCTTCGTCATCACTACATTATTTCAATTAAATAC120.10869565217391304No Hit
GGCACTAGAATGGACCCTTCCTCATCACTACATTATTTCAATTAAATACA120.10869565217391304No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATGT120.10869565217391304No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)