FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151866

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151866
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12754
Total Bases1.8 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC520440.80288536929591No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC260420.417124039517017No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC151211.85510428100988No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG3022.367884585228164No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC1961.5367727771679474No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA1441.1290575505723695No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA1050.823271130625686No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG740.5802101301552455No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA670.525325388113533No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA650.5096440332444724No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA610.478281323506351No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA520.4077152265955779No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA500.39203387172651716No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA480.37635251685745646No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA470.36851183942292615No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG400.31362709738121375No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA380.29794574251215306No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA360.28226438764309236No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA360.28226438764309236No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT300.2352203230359103No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA290.22737964560137994No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA260.20385761329778895No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA260.20385761329778895No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA250.19601693586325858No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTTT230.18033558099419789No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA220.17249490355966754No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA210.1646542261251372No Hit
GGCACTAGAATGGACCCTTCCTCATCACTACATTATTTCAATTAAATACA200.15681354869060687No Hit
GGCACTAGAATGGACCTCTCCCTCATCACTACATTATTTCAATTAAATAC170.13329151638701583No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA160.1254508389524855No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT160.1254508389524855No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT140.10976948408342481No Hit
GGCACTAGAATGGACCTCCTCCTCATCACTACATTATTTCAATTAAATAC140.10976948408342481No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTACATTATTTCAATTAAATAC140.10976948408342481No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT140.10976948408342481No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA140.10976948408342481No Hit
GGCACTAGAATGGCCTCTTCCTCATCACTACATTATTTCAATTAAATACA140.10976948408342481No Hit
GGCACTAGAATGGACCTCTTCGTCATCACTACATTATTTCAATTAAATAC130.10192880664889448No Hit
GGCACTAGAATGGACCTCTTCCCCATCACTACATTATTTCAATTAAATAC130.10192880664889448No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)