FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151884

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151884
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10062
Total Bases1.4 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC467946.50168952494534No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC205220.39355992844365No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG4354.3231961836613No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC2252.23613595706619No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA1611.6000795070562512No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG1281.2721129000198768No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA1081.073345259391771No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC930.9242695289206917No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA690.6857483601669648No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA620.6161796859471278No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA520.516795865633075No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT490.48698071953885913No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG470.4671039554760485No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA450.4472271914132379No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA390.3875968992248062No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA370.36772013516199564No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA370.36772013516199564No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA320.3180282250049692No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT310.3080898429735639No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA300.2981514609421586No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT290.2882130789107533No Hit
GGCACTAGAATGGACCCTTCCTCATCACTACATTATTTCAATTAAATACA240.2385211687537269No Hit
GGCACTAGAATGGCCTCTTCCTCATCACTACATTATTTCAATTAAATACA240.2385211687537269No Hit
GGCACTAGAATGGACCTCTCCCTCATCACTACATTATTTCAATTAAATAC170.16895249453388989No Hit
GGCACTAGAATGGACCTCTTCCTCATAACTACATTATTTCAATTAAATAC170.16895249453388989No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT160.1590141125024846No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT160.1590141125024846No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTACATTATTTCAATTAAATAC150.1490757304710793No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTTT150.1490757304710793No Hit
GGCACTAGAATGGACCCCTTCCTCATCACTACATTATTTCAATTAAATAC140.139137348439674No Hit
AAATACCGTTCGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT140.139137348439674No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCTTCCATGTACTCTGC140.139137348439674Clontech SMARTer II A Oligonucleotide (95% over 22bp)
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGACCC130.12919896640826875No Hit
GGCACTAGAATGGACCTCTTCCCCATCACTACATTATTTCAATTAAATAC120.11926058437686345No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACGTTATTTCAATTAAATAC120.11926058437686345No Hit
GGCACTAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACAA110.10932220234545817No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACATTACGCTGTAGAA110.10932220234545817No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)