FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151886

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151886
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10617
Total Bases1.5 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC502047.28265988508995No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC271525.572195535461994No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG3653.4378826410473766No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA1471.3845719129697656No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG960.9042102288782142No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA850.8006028068192521No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA650.6122256758029575No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA530.4991993971931808No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA510.4803616840915513No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA470.44268625788829236No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA440.4144296882358482No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA410.386173118583404No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTTT400.3767542620325892No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG390.3673354054817745No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA340.32024112272770083No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA320.3014034096260714No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT310.29198455307525667No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA260.24489027032118302No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT250.23547141377036826No Hit
GGCACTAGAATGGACCTCTTCGTCATCACTACATTATTTCAATTAAATAC240.22605255721955356No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT210.19779598756710934No Hit
AAATACCGTTCAAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT210.19779598756710934No Hit
GGCACTAGAATGGACCCTTCCTCATCACTACATTATTTCAATTAAATACA200.1883771310162946No Hit
GGCACTAGAATGGACCTCTCCCTCATCACTACATTATTTCAATTAAATAC190.17895827446547988No Hit
GGCACTAGAATGGACCTCTTCCCCATCACTACATTATTTCAATTAAATAC190.17895827446547988No Hit
AAATACCGTTCAGGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT160.1507017048130357No Hit
GGCACTAGAATGGACCTCCTCCTCATCACTACATTATTTCAATTAAATAC150.14128284826222096No Hit
AAATACCGTTCAGAGCTGCCCCCAGGAGCTTGCAAATTGCTGCTGGAACC140.13186399171140623No Hit
AAATACCGTTCAGAGCGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT140.13186399171140623No Hit
GGCACTAGAATGGACCTCTTCCTCATCACCACATTATTTCAATTAAATAC130.12244513516059151No Hit
AAATACCGTTAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT130.12244513516059151No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTACATTATTTCAATTAAATAC130.12244513516059151No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT130.12244513516059151No Hit
GGCACTAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACAA120.11302627860977678No Hit
GGCACTAGAATGGCCTCTTCCTCATCACTACATTATTTCAATTAAATACA120.11302627860977678No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCCGGAACC120.11302627860977678No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATGC110.10360742205896205No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACGTTATTTCAATTAAATAC110.10360742205896205No Hit
GGCACTAGAATGGACCTCTTACTCATCACTACATTATTTCAATTAAATAC110.10360742205896205No Hit
AAATACCGTTCGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT110.10360742205896205No Hit
GGCACTAGAATGGACCTCTTCCTCATAACTACATTATTTCAATTAAATAC110.10360742205896205No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCAGCTGGAACC110.10360742205896205No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGATTGCAAATTGCTGCTGGAACC110.10360742205896205No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT110.10360742205896205No Hit
AAATACCGTTCAGAGCTGCCACCAGGGGCTTGCAAATTGCTGCTGGAACC110.10360742205896205No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)