FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151888

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151888
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13513
Total Bases1.9 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC475435.18093687560127No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC186813.82372530156146No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC173312.824687338118848No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC145610.774809442758825No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG4303.1821209205949823No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA1270.9398357137571228No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG1070.7918300895434027No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA980.7252275586472285No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT630.46621771627321834No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT520.3848146229556723No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA510.3774143417449863No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG490.36261377932361427No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA490.36261377932361427No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA450.33301265448087025No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA410.30341152963812623No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA390.2886109672167543No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA330.2442092799526382No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA300.2220084363205802No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA290.21460815510989417No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT290.21460815510989417No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA280.20720787389920817No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA260.19240731147783616No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT240.17760674905646415No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTTT240.17760674905646415No Hit
GGCACTAGAATGGACCCTTCCTCATCACTACATTATTTCAATTAAATACA220.16280618663509214No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTACATTATTTCAATTAAATAC210.15540590542440613No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA210.15540590542440613No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA200.1480056242137201No Hit
GGCACTAGAATGGCCTCTTCCTCATCACTACATTATTTCAATTAAATACA200.1480056242137201No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA190.14060534300303412No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGACCC180.1332050617923481No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA170.1258047805816621No Hit
GGCACTAGAATGGACCTCTTCCCCATCACTACATTATTTCAATTAAATAC170.1258047805816621No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA160.1184044993709761No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT160.1184044993709761No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA160.1184044993709761No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACATTACGCTGTAGAA150.1110042181602901No Hit
AAATACCGTTCAGAGCTGCCCCCAAGAGCTTGCAAATTGCTGCTGGAACC140.10360393694960408No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGACCC140.10360393694960408No Hit
GGCACTAGAATGGACCTCTTACTCATCACTACATTATTTCAATTAAATAC140.10360393694960408No Hit
GGCACTAGAATGGACCTCTCCCTCATCACTACATTATTTCAATTAAATAC140.10360393694960408No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)