FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151892

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151892
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12689
Total Bases1.8 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC609448.025849160690356No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC275121.68019544487351No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC7926.241626605721491No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG3362.647962802427299No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA1581.245172984474742No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA1250.9851052092363465No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG980.7723224840412957No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA730.5753014421940263No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA680.5358972338245724No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA570.449207975411774No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA500.3940420836945385No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT500.3940420836945385No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA460.36251871699897553No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG420.3309953503034124No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA390.30735282528174007No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA380.2994719836078493No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA370.2915911419339585No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA290.2285444085428324No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA280.22066356686894162No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTACATTATTTCAATTAAATAC280.22066356686894162No Hit
GGCACTAGAATGGACCCTTCCTCATCACTACATTATTTCAATTAAATACA230.18125935849948777No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTTT230.18125935849948777No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT190.14973599180392466No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA170.1339743084561431No Hit
AAATACCGTTCAAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT160.12609346678225233No Hit
GGCACTAGAATGGCCTCTTCCTCATCACTACATTATTTCAATTAAATACA160.12609346678225233No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT150.11821262510836156No Hit
AAATACCGTTCAGAGCGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT150.11821262510836156No Hit
GGCACTAGAATGGACCTCTCCCTCATCACTACATTATTTCAATTAAATAC150.11821262510836156No Hit
GGCACTAGAATGGACCTCTTCCCCATCACTACATTATTTCAATTAAATAC130.10245094176058002No Hit
GGCACTAGAATGGACCTCTGCGTTGATACCACTGCCTTGTTTTTAAGTTT130.10245094176058002Clontech SMARTer II A Oligonucleotide (95% over 22bp)
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTAAATTAAATAC130.10245094176058002No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)