FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151894

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151894
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12995
Total Bases1.8 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC772359.43055021161986No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC10748.264717198922662No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC9517.3181993074259335No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG2531.9469026548672566No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA1831.4082339361292806No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA1561.2004617160446325No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA970.7464409388226241No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA970.7464409388226241No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA900.6925740669488265No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA840.6464024624855713No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA690.5309734513274336No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA620.477106579453636No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA600.461716044632551No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG600.461716044632551No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA520.4001539053482109No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA480.3693728357060408No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTTT330.25394382454790304No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA320.2462485571373605No Hit
GGCACTAGAATGGACCTCTTCCCCATCACTAAATTATTTCAATTAAATAC290.22316275490573298No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTAAATTATTTCAATTAAATAC290.22316275490573298No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA290.22316275490573298No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG270.20777222008464794No Hit
GGCACTAGAATGGCCTCTTCCTCATCACTAAATTATTTCAATTAAATACA230.17699115044247787No Hit
GGCACTAGAATGGACCCCTTCCTCATCACTAAATTATTTCAATTAAATAC220.16929588303193538No Hit
GGCACTAGAATGGACCTCTCCCTCATCACTAAATTATTTCAATTAAATAC210.16160061562139283No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT200.15390534821085033No Hit
GGCACTAGAATGGACCCTTCCTCATCACTAAATTATTTCAATTAAATACA200.15390534821085033No Hit
GGCACTAGAATGGACCTCTTCCTCACCACTAAATTATTTCAATTAAATAC190.1462100808003078No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT190.1462100808003078No Hit
GGCACTAGAATGGACCTCTTCGTCATCACTAAATTATTTCAATTAAATAC150.11542901115813775No Hit
GGCACTAGAATGGACTTCTTCCTCATCACTAAATTATTTCAATTAAATAC150.11542901115813775No Hit
GACACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC140.10773374374759521No Hit
AAATACCGTTCGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT130.10003847633705272No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTAAATTAAATAC130.10003847633705272No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)