FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151898

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151898
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13185
Total Bases1.9 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC392829.791429654910885No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC390929.647326507394766No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC163412.392870686386043No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC3942.988244216913159No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG2942.229806598407281No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA1190.9025407660219947No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA1070.8115282518012893No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA930.7053469852104665No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG830.6295032233598787No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA740.5612438376943496No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA550.41714069017823285No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA520.3943875616230565No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA480.3640500568828214No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA470.3564656806977626No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA430.3261281759575275No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA380.2882062950322336No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA350.26545316647705725No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA350.26545316647705725No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA310.23511566173682213No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT310.23511566173682213No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG310.23511566173682213No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTTT310.23511566173682213No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA290.21994690936670458No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA280.2123625331816458No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA270.20477815699658702No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA230.1744406522563519No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT220.16685627607129314No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA220.16685627607129314No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA210.15927189988623436No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA200.15168752370117558No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA190.1441031475161168No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA160.12135001896094046No Hit
GGCACTAGAATGGACCTCTCCCTCATCACTACATTATTTCAATTAAATAC150.11376564277588168No Hit
GGCACTAGAATGGCCTCTTCCTCATCACTAAATTATTTCAATTAAATACA140.1061812665908229No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)