FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151914

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151914
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10577
Total Bases1.5 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC338331.98449465822067No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC181917.19769310768649No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC120511.392644417131512No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC110910.485014654438876No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG3833.6210645740758247No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG1020.9643566228609247No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA970.9170842393873498No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA880.8319939491349154No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT540.5105417415146072No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA490.4632693580410324No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA480.4538148813463175No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA470.4443604046516026No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA440.4159969745674577No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT430.40654249787274277No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG400.3781790677885979No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA390.36872459109388295No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA370.34981563770445306No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA310.2930887775361634No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA310.2930887775361634No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA280.26472534745201853No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT240.22690744067315874No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA200.18908953389429894No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA200.18908953389429894No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA200.18908953389429894No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA170.1607261038101541No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA170.1607261038101541No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA170.1607261038101541No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA170.1607261038101541No Hit
GGCACTAGAATGGACCTCTTCGTCATCACTACATTATTTCAATTAAATAC150.1418171504207242No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA150.1418171504207242No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGACCC140.13236267372600927No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT140.13236267372600927No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTTT140.13236267372600927No Hit
GGCACTAGAATGGACCTCTCCCTCATCACTACATTATTTCAATTAAATAC130.12290819703129433No Hit
AAATACCGTTCAAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT120.11345372033657937No Hit
GGCACTAGAATGGACCCTTCCTCATCACTACATTATTTCAATTAAATACA120.11345372033657937No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTACATTATTTCAATTAAATAC120.11345372033657937No Hit
GGCACTAGAATGGACCCCTTCCTCATCACTACATTATTTCAATTAAATAC120.11345372033657937No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT120.11345372033657937No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)