FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151928

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151928
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11368
Total Bases1.6 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC251122.088318085855033No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC230720.293807178043632No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC194017.065446868402535No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC152913.450035186488387No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG3903.4306826178747363No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA860.7565095003518649No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG830.7301196340605207No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA640.5629838142153413No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA560.49261083743842365No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA540.4750175932441942No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG380.3342716396903589No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA370.3254750175932442No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA340.29908515130190005No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA330.2902885292047854No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA320.28149190710767064No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT320.28149190710767064No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA290.25510204081632654No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA290.25510204081632654No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA290.25510204081632654No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA280.24630541871921183No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT250.21991555242786767No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA250.21991555242786767No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA210.18472906403940886No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA210.18472906403940886No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTTT200.17593244194229415No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA170.14954257565095003No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA170.14954257565095003No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT160.14074595355383532No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA150.1319493314567206No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTACATTATTTCAATTAAATAC150.1319493314567206No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT150.1319493314567206No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA140.12315270935960591No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGACCC140.12315270935960591No Hit
AAATACCGTTCAAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT130.1143560872624912No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA130.1143560872624912No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGACCC130.1143560872624912No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT120.1055594651653765No Hit
AAATACCGTTCAGAGCTGCCCCCAAGAGCTTGCAAATTGCTGCTGGAACC120.1055594651653765No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACATTACGCTGTAGAA120.1055594651653765No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)