FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151932

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151932
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8288
Total Bases1.2 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC317338.28426640926641No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC233328.14913127413127No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC4175.031370656370656No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG2913.511100386100386No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA931.122104247104247No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG891.0738416988416988No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA640.7722007722007722No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG590.7118725868725868No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA490.5912162162162162No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTTT380.4584942084942085No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA280.33783783783783783No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA270.32577220077220076No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA250.3016409266409266No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT210.2533783783783784No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA210.2533783783783784No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA210.2533783783783784No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT210.2533783783783784No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA190.22924710424710426No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA180.2171814671814672No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGACCC160.19305019305019305No Hit
AAATACCGTTCAAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT160.19305019305019305No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTACATTATTTCAATTAAATAC140.16891891891891891No Hit
AAATACCGTTCAGAGCGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT140.16891891891891891No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC140.16891891891891891No Hit
AAATCCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT130.15685328185328185No Hit
AAATACCGTTCGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT130.15685328185328185No Hit
GGCACTAGAATGGACCTCTCCCTCATCACTACATTATTTCAATTAAATAC130.15685328185328185No Hit
GGCACTAGAATGGACCTCTTCCCCATCACTACATTATTTCAATTAAATAC120.14478764478764478No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA110.1327220077220077No Hit
GGCACTAGAATGGACCCTTCCTCATCACTACATTATTTCAATTAAATACA110.1327220077220077No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTGCATTATTTCAATTAAATAC110.1327220077220077No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT110.1327220077220077No Hit
AAATACCGTTAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT100.12065637065637065No Hit
AAATACCGTTCAGAGCTGCCGCCAGGAGCTTGCAAATTGCTGCTGGAACC100.12065637065637065No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTCGCAAATTGCTGCTGGAACC100.12065637065637065No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT100.12065637065637065No Hit
GGCACTAGAATGGACCTCTTCGTCATCACTACATTATTTCAATTAAATAC90.1085907335907336No Hit
AAATACCGTTCAGAGCTGCCACCAGGGGCTTGCAAATTGCTGCTGGAACC90.1085907335907336No Hit
AAATACCGTTCAGAGCTGCCCCCAGGAGCTTGCAAATTGCTGCTGGAACC90.1085907335907336No Hit
GGCACTAGAATGGACCTCTCCTCATCACTACATTATTTCAATTAAATACA90.1085907335907336No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGATTGCAAATTGCTGCTGGAACC90.1085907335907336No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)