FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151944

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151944
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10114
Total Bases1.4 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC396339.18331026300178No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC210120.77318568321139No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC10009.887284951552305No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG4744.686573067035791No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG1381.364445323314218No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA1221.206248764089381No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA800.7909827961241842No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA720.7118845165117659No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA550.5438006723353767No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA540.5339133873838244No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA490.4844769626260629No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG470.4647023927229583No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT450.44492782281985366No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA390.3856041131105398No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT390.3856041131105398No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA360.35594225825588294No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT330.32628040340122605No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC300.2966185485465691No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA290.2867312635950168No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTTT220.2175202689341507No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA210.20763298398259838No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA200.19774569903104605No Hit
GGCACTAGAATGGACCCTTCCTCATCACTACATTATTTCAATTAAATACA200.19774569903104605No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTACATTATTTCAATTAAATAC200.19774569903104605No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT190.18785841407949377No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT180.17797112912794147No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGACCC180.17797112912794147No Hit
GGCACTAGAATGGACCTCTCCCTCATCACTACATTATTTCAATTAAATAC180.17797112912794147No Hit
GGCACTAGAATGGACCTCTTCGTCATCACTACATTATTTCAATTAAATAC170.16808384417638916No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT130.12853470437017994No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTAAATTAAATAC130.12853470437017994No Hit
AAATACCGTTCAGAGCGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT120.11864741941862765No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTTTACGCTGTAGAACCATGT110.10876013446707535No Hit
GGCACTAGAATGGACCTCTTCCTCACCACTACATTATTTCAATTAAATAC110.10876013446707535No Hit
AAATACCGTTCGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT110.10876013446707535No Hit
GGCACTAGAATGGACTTCTTCCTCATCACTACATTATTTCAATTAAATAC110.10876013446707535No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCTTCCATGTACTCTGC110.10876013446707535Clontech SMARTer II A Oligonucleotide (95% over 22bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)