FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151963

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151963
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7715
Total Bases1.1 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT434456.305897602073884No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG168421.827608554763447No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTCCTCTAAGACCAAGTCTC2713.512637718729747No Hit
ACTTAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC1471.9053791315618924No Hit
ACTTAAAAACAAGGCAGTGCTGTGCCCTCCCAGGTACCCCAAGCTGGCAG1051.3609850939727801No Hit
ACTTAAAAACAAGGCAGTGCTATGCCCTCCCAGGTACCCCAAGCTGGCAG640.8295528191834091No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTCCTCTAAGACCAAGTCTC570.7388204795852237No Hit
CTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC510.661049902786779No Hit
ACTTAAAAACAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC400.5184705119896306No Hit
ACTTAAAAACAAGGCAGTGCTGAACGGTATTTCCTCTAAGACCAAGTCTC290.37589112119248214No Hit
ACTTAAAAAAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC270.34996759559300067No Hit
ACTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC250.32404406999351915No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCCGCCT160.20738820479585227No Hit
ACTTAAAAACAAGGAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC160.20738820479585227No Hit
ACTTAAAAACAAGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC140.18146467919637072No Hit
ACTTAAAAACAAGGCAGTGCTAATGACTAATGGTGCTACAGTTTCTGCCT140.18146467919637072No Hit
ATTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC130.16850291639662995No Hit
ACTTAAAAACAAGGCGGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT130.16850291639662995No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGCGCTACAGTTTCTGCCT120.15554115359688916No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGCTTCTGCCT120.15554115359688916No Hit
ACTTAAAAACAAGGCAGTGCTATCAACGAAGAGGTCCATTCTAGTGCCCC120.15554115359688916No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTCTCTGCCT110.1425793907971484No Hit
TGTGTGTGTGTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC110.1425793907971484No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAGG100.12961762799740764No Hit
TGTGTGTGTGTTTCTGTGGCAGCTCTGAACGGTATTTCCTCTAAGACCAA100.12961762799740764No Hit
ACTTAAAAACAAGGCAGTGCTAATACCTAATGGTGCTACAGTTTCTGCCT100.12961762799740764No Hit
ACTTAAAAACAAGGCAGTGTTAATGCCTAATGGTGCTACAGTTTCTGCCT100.12961762799740764No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTGCAGTTTCTGCCT90.1166558651976669No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCCAATGGTGCTACAGTTTCTGCCT90.1166558651976669No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGTCT90.1166558651976669No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCC90.1166558651976669No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGTTACAGTTTCTGCCT80.10369410239792613No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)