FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151964

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151964
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7715
Total Bases1.1 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC406452.67660401814646No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC142618.483473752430328No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG2703.4996759559300066No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT1051.3609850939727801No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT650.8425145819831497No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG560.7258587167854829No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT550.712896953985742No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA530.6869734283862605No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT370.47958522359040834No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGACCC340.440699935191186No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA290.37589112119248214No Hit
AAATACCGTTCAAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT280.36292935839274143No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTTTACGCTGTAGAACCATG270.34996759559300067No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA230.2981205443940376No Hit
AAATACCGTTAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT210.2721970187945561No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT210.2721970187945561No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA200.2592352559948153No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA190.24627349319507452No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA180.2333117303953338No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA160.20738820479585227No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGATGCTGGAACC160.20738820479585227No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA150.19442644199611148No Hit
AAATACCGTTCAGAGCTGCCCCCAGGAGCTTGCAAATTGCTGCTGGAACC140.18146467919637072No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA140.18146467919637072No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGTAAATTGCTGCTGGAACC130.16850291639662995No Hit
AAATACCGTTCGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT130.16850291639662995No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCCTGCAAATTGCTGCTGGAACC130.16850291639662995No Hit
AAATACCGTTCAGAGCGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT130.16850291639662995No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAAAC120.15554115359688916No Hit
AAATACCGTTCAGACTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT120.15554115359688916No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTTT120.15554115359688916No Hit
AAATCCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT110.1425793907971484No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGGACC110.1425793907971484No Hit
AAATACCTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT110.1425793907971484No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTTCTGCTGGAACC110.1425793907971484No Hit
AAATACCGTTCAGAGCTGCCACCAGGGGCTTGCAAATTGCTGCTGGAACC110.1425793907971484No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA100.12961762799740764No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACATTACGCTGTAGAA100.12961762799740764No Hit
AAAACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT100.12961762799740764No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA100.12961762799740764No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATCGCTGCTGGAACC100.12961762799740764No Hit
AAATACCGTTCAGAGCTGCCACCGGGAGCTTGCAAATTGCTGCTGGAACC100.12961762799740764No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCCGGAACC100.12961762799740764No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGCACC90.1166558651976669No Hit
AAATACCGTTCAGAGCTGCCGCCAGGAGCTTGCAAATTGCTGCTGGAACC90.1166558651976669No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGTTTGCAAATTGCTGCTGGAACC80.10369410239792613No Hit
AAATACCGTTAAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC80.10369410239792613No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACA80.10369410239792613No Hit
AAATACCGTTCAGAGCTGCCACCAGGATCTTGCAAATTGCTGCTGGAACC80.10369410239792613No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGATTGCAAATTGCTGCTGGAACC80.10369410239792613No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)